Britta Wlotzka

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Spiegelmers are high-affinity l-enantiomeric oligonucleotide ligands that display high resistance to enzymatic degradation compared with d-oligonucleotides. The target binding properties of Spiegelmers can be designed by an in vitro-selection process starting from a random pool of oligonucleotides. Applying this method, a Spiegelmer with high affinity (K(D)(More)
Employing in vitro selection techniques, we have generated biostable RNA-based compounds, so-called Spiegelmers, that specifically bind n-octanoyl ghrelin, the recently discovered endogenous ligand for the type 1a growth hormone secretagogue (GHS) receptor. Ghrelin is a potent stimulant of growth hormone release, food intake, and adiposity. We demonstrate(More)
Single-stranded mirror-image oligonucleotides (Spiegelmers) are highly resistant to nuclease degradation and are capable of tightly and specifically binding to protein targets. Here we explored the potential of Spiegelmers as in vivo imaging probes for positron emission tomography (PET). We investigated the biodistribution and pharmacokinetics of(More)
The search for minimal chemical systems which are capable to self-replicate is of considerable interest within the context of early biochemical evolution (Joyce, 1987). We have shown that the formation of a hexadeoxynucleotide 3 (R 1 = CH 3, L = p) from two trideoxynucleotides 1 (R 1 = CH3) and 2 (R 2 = H) in the presence of the water-soluble(More)
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