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We report on the presence of a functional hydroxyectoine biosynthesis gene cluster, ectABCD-ask, in Pseudomonas stutzeri DSM5190(T) and evaluate the suitability of P. stutzeri DSM5190(T) for hydroxyectoine production. Furthermore, we present information on heterologous de novo production of the compatible solute hydroxyectoine in Escherichia coli. In this(More)
The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, and biochemical approaches to address(More)
In this study we report the presence of a functional hydroxyectoine biosynthesis gene cluster ectABCD/ask in Pseudomonas stutzeri type strain DSM5190 T and evaluate the suitability of P. stutzeri DSM5190 T for hydroxyectoine production. Furthermore we present heterologous de novo production of the compatible solute hydroxyectoine in Escherichia coli. In(More)
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