Brinda Prasad

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P450 enzymes are known for catalyzing hydroxylation reactions of non-activated C-H bonds. For example, P450cam from Pseudomonas putida oxidizes (1R)-(+)-camphor to 5-exo-hydroxy camphor and further to 5-ketocamphor. This hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen (O2) is coupled to the oxidation of the(More)
This paper presents a high throughput screening algorithm for leukemia cells that has been designed, implemented, and tested. It performs a recursive image segmentation technique, row-wise and column-wise, on edge detected cell image. The recursive image segmentation successfully eliminates background pixels from foreground pixels by only segmenting image(More)
P450(cam) (CYP101A1) is a bacterial monooxygenase that is known to catalyze the oxidation of camphor, the first committed step in camphor degradation, with simultaneous reduction of oxygen (O2). We report that P450(cam) catalysis is controlled by oxygen levels: at high O2 concentration, P450(cam) catalyzes the known oxidation reaction, whereas at low O2(More)
C.elegans offers the promise of understanding the mechanisms underlying animal behavior at the molecular and cellular levels. This paper presents an application-specific, complete quantification system to obtain population statistics of Caenorhabditis elegans (C. elegans) eggs that is accurate and fast compared to currently used manual techniques. The(More)
A low-abundance form of water, H(2)(17)O, was enriched from 0.04% to ∼90% by slow evaporation and fractional distillation of tap water. The density and refractive index for H(2)(17)O are reported. Gas chromatography-mass spectrometry (GC-MS) of (16)O- and (17)O-1-hexanols and their trimethyl silyl ethers and of (16)O- and (17)O-hexamethyl disiloxanes was(More)
Cytochrome P450cam (a camphor hydroxylase) from the soil bacterium Pseudomonas putida shows potential importance in environmental applications such as the degradation of chlorinated organic pollutants. Seven P450cam mutants generated from Sequence Saturation Mutagenesis (SeSaM) and isolated by selection on minimal media with either 3-chloroindole or the(More)
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