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High resolution mapping of mast cell membranes reveals primary and secondary domains of FcεRI and LAT

• Bridget S. Wilson, Janet R. Pfeiffer, Zurab Surviladze, Elizabeth A. Gaudet, Janet M. Oliver
• The Journal of cell biology
• 2001

In mast cells, cross-linking the high-affinity IgE receptor (Fc(epsilon)RI) initiates the Lyn-mediated phosphorylation of receptor ITAMs, forming phospho-ITAM binding sites for Syk. Previous… (More)

A Rab1 mutant affecting guanine nucleotide exchange promotes disassembly of the Golgi apparatus

• Bridget S. Wilson, Claude Nuoffer, +4 authors Marilyn Gist Farquhar
• The Journal of cell biology
• 1994

The Golgi apparatus is a dynamic organelle whose structure is sensitive to vesicular traffic and to cell cycle control. We have examined the potential role for rab1a, a GTPase previously associated… (More)

Increased protein expression through improved ribosome-binding sites obtained by library mutagenesis.

• Bridget S. Wilson, C R Kautzer, Douglas Antelman
• BioTechniques
• 1994

This report describes a method whereby library mutagenesis combined with drug selection was used to generate unique and efficient ribosome-binding sites (RBS) for expressing recombinant proteins in… (More)

Increased antibody expression from Escherichia coli through wobble-base library mutagenesis by enzymatic inverse PCR.

• Willem Pim C Stemmer, Shaunna K Morris, C R Kautzer, Bridget S. Wilson
• Gene
• 1993

We tested the value of a new library mutagenesis approach, called library enzymatic inverse PCR (LEIPCR), for expression-level enhancement of antibody Fv fragments produced in Escherichia coli. The… (More)

Selection of an active single chain Fv antibody from a protein linker library prepared by enzymatic inverse PCR.

• Willem P. C. Stemmer, Shaunna K Morris, Bridget S. Wilson
• BioTechniques
• 1993

Enzymatic inverse PCR mutagenesis was developed as a simple and reliable method for the construction of large libraries of site-directed mutants. Enzymatic inverse PCR library mutagenesis uses a… (More)

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