Brian Markoff

Learn More
Three monoclonal antibodies (MAbs), E4, L1-4, and L1-24, to the major outer membrane protein (MOMP) of Chlamydia trachomatis were identified that neutralized in vitro the infectivity of members of the B- and C-related complex as well as the mouse pneumonitis strain. MAbs L1-4, L1-24, and E4 gave a strong signal in an indirect immunofluorescence assay and/or(More)
An L2 serovar of Chlamydia trachomatis, isolated from a patient with proctocolitis, has been identified that does not contain the common C. trachomatis 7.5-kb plasmid. This isolate was propagated in vitro showing that this plasmid is not required for the growth of C. trachomatis.
The major outer membrane protein (MOMP) of C. trachomatis is surface exposed and accounts for 60% of the outer membrane protein of this widespread pathogen. This protein has been shown to contain conserved regions among the 15 serovars of C. trachomatis as well as variable domains (VD) that are partially responsible for antigenic differences among the(More)
DNA from Chlamydia trachomatis serovars L3, C, and E corresponding to the open reading frames of the 60-kDa protein and of a putative 15-kDa protein was sequenced. The open reading frames coding for the 60-kDa protein had 1,641 bp in the three serovars. Compared with the L3 serovar, there were 9 and 11 amino acid changes in the C and E serovars,(More)
Numerous epidemiological studies have linked tobacco use with a wide variety of diseases. Strong government-supported anti-smoking campaigns have decreased the prevalence of smoking in many developed countries, including the United States. However, it remains a major public health pariah. In developing countries, such as China, cigarette smoking and other(More)
As the cost of care rises and fragmentation of health care increases, care transitions have become critical parts of the health care system. Physicians and other inpatient providers have the responsibility to communicate to subsequent providers, but such communication occurs far less than is optimal. Timely discharge summaries for the next-level provider,(More)
Inadequate communication as patients transition across venues of care carries substantial risks. At the time of discharge, changes in medications may be missed, abnormal or pending test results ignored, and evolving aspects of the patient’s condition may not receive appropriate attention. The essential nature of timely communication at discharge has been(More)
Asthma continues to be a challenging disease to treat in both the inpatient and outpatient settings. The growing database on therapeutic interventions at the time of transition from the acute to chronic phase of this disease is encouraging. Glucocorticoids and inhaled beta-agonists clearly reduce readmission and relapse. Other medications and educational(More)
Sera from seven patients from whom a C. trachomatis serovar L2 strain was isolated were tested in vitro for their ability to neutralize the infectivity of this organism. In one patient an inguinal lymph node was culture positive, whereas the remaining six patients had positive rectal biopsies. Sera from four of the patients, including the patient with the(More)