Brian MM Ahmer

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The contribution of a gene to the fitness of a bacterium can be assayed by whether and to what degree the bacterium tolerates transposon insertions in that gene. We use this fact to compare the fitness of syntenic homologous genes among related Salmonella strains and thereby reveal differences not apparent at the gene sequence level. A transposon Tn5(More)
BACKGROUND The type III secretion system (TTSS) is an important virulence determinant of Gram-negative bacterial pathogens. It enables the injection of effector proteins into the cytosol of eukaryotic cells. These effectors ultimately manipulate the cellular functions of the infected organism. Salmonella enterica serovar Typhimurium encodes two virulence(More)
It is an amazing time to be a microbiologist. We have progressed from the study of microbes in monoculture to the study of microbes in mixed culture to the study of complex microbial communities in natural environments. Colony purification was an extremely powerful reductionist tool, but after decades of genetic analyses, many genes of model microbes still(More)
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