Brian M. Taff

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We present the first known implementation of a passive, scalable architecture for trapping, imaging, and sorting individual microparticles, including cells, using a positive dielectrophoretic (p-DEP) trapping array. Our array-based technology enables "active coverslips" where, when scaled, many individually held cells can be sorted based upon imaged spatial(More)
We show the application of a commercially available photopatternable silicone (PPS) that combines the advantageous features of both PDMS and SU-8 to address a critical bioMEMS materials deficiency. Using PPS, we demonstrate the ability to pattern free-standing mechanically isolated elastomeric structures on a silicon substrate: a feat that is challenging to(More)
Attempts to regulate and monitor the roles of cell-cell interactions in engineered multicellular constructs have motivated numerous approaches to cell patterning. We present here a stencil-delineated electroactive patterning (S-DEP) that combines dielectrophoresis (DEP) and stencil patterning to create cell clusters with customizable shapes, positions, and(More)
We present quantitative modeling software for simulating multiple forces acting on a single particle in a microsystem. In this paper, we focus on dielectrophoretic (DEP) trapping of single cells against fluid flow. The software effectively models the trapping behavior for a range of particles including beads, mammalian cells, viruses, and bacteria. In(More)
We offer the first known platform for parallelized single-cell manipulation that combines negative dielectrophoretic (n-DEP) sorting with the efficient loading behavior of hydrodynamic traps. Our devices provide manipulations using ejection-and/or exclusion-based techniques. In ejection operations we unload targeted sites by driving their associated(More)
Here we show the application of a commercially available photopatternable silicone (PPS) that combines advantages of both PDMS and SU-8 to address a critical need in material building blocks for bioMEMS. Using PPS we have demonstrated the ability to pattern free-standing mechanically isolated elastomeric structures on a silicon substrate, a feat challenging(More)
We present a platform for parallelized manipulations of individual polarizable micron-scale particles (i.e., microparticles) that combines negative dielectrophoretic forcing with the passive capture of hydrodynamic weir-based trapping. Our work enables manipulations using ejection- andor exclusion-based methods. In ejection operations, we unload targeted(More)
This project focuses on the development of a microorganism concentrator. Pathogen detection, particularly MEMS based detection, is often limited by sample concentration. The proposed concentrator will interface with a pathogen detector. This type of pathogen concentrator can be useful for many kinds of applications including water purification systems,(More)
Our group performs research on BioMEMS, applying microfabrication technology to illuminate biological systems, especially at the cellular level. Specifically, we develop technologies that are used to manipulate cells or make measurements from them. Our research builds upon various disciplines: electrical engineering, microfabrication, bioengineering,(More)