Brian M. Taff

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We present the first known implementation of a passive, scalable architecture for trapping, imaging, and sorting individual microparticles, including cells, using a positive dielectrophoretic (p-DEP) trapping array. Our array-based technology enables "active coverslips" where, when scaled, many individually held cells can be sorted based upon imaged spatial(More)
We show the application of a commercially available photopatternable silicone (PPS) that combines the advantageous features of both PDMS and SU-8 to address a critical bioMEMS materials deficiency. Using PPS, we demonstrate the ability to pattern free-standing mechanically isolated elastomeric structures on a silicon substrate: a feat that is challenging to(More)
Attempts to regulate and monitor the roles of cell-cell interactions in engineered multicellular constructs have motivated numerous approaches to cell patterning. We present here a stencil-delineated electroactive patterning (S-DEP) that combines dielectrophoresis (DEP) and stencil patterning to create cell clusters with customizable shapes, positions, and(More)
We present quantitative modeling software for simulating multiple forces acting on a single particle in a microsystem. In this paper, we focus on dielectrophoretic (DEP) trapping of single cells against fluid flow. The software effectively models the trapping behavior for a range of particles including beads, mammalian cells, viruses, and bacteria. In(More)
We offer the first known platform for parallelized single-cell manipulation that combines negative dielectrophoretic (n-DEP) sorting with the efficient loading behavior of hydrodynamic traps. Our devices provide manipulations using ejection-and/or exclusion-based techniques. In ejection operations we unload targeted sites by driving their associated(More)
Here we show the application of a commercially available photopatternable silicone (PPS) that combines advantages of both PDMS and SU-8 to address a critical need in material building blocks for bioMEMS. Using PPS we have demonstrated the ability to pattern free-standing mechanically isolated elastomeric structures on a silicon substrate, a feat challenging(More)
We present a platform for parallelized manipulations of individual polarizable micron-scale particles (i.e., microparticles) that combines negative dielectrophoretic forcing with the passive capture of hydrodynamic weir-based trapping. Our work enables manipulations using ejection- andor exclusion-based methods. In ejection operations, we unload targeted(More)
This project focuses on the development of a microorganism concentrator. Pathogen detection, particularly MEMS based detection, is often limited by sample concentration. The proposed concentrator will interface with a pathogen detector. This type of pathogen concentrator can be useful for many kinds of applications including water purification systems,(More)