Brian M. Green

Learn More
Eukaryotic cells use numerous mechanisms to ensure that no segment of their DNA is inappropriately re-replicated, but the importance of this stringent control on genome stability has not been tested. Here we show that re-replication in Saccharomyces cerevisiae can strongly induce the initial step of gene amplification, increasing gene copy number from one(More)
To maintain genome stability, the entire genome of a eukaryotic cell must be replicated once and only once per cell cycle. In many organisms, multiple overlapping mechanisms block rereplication, but the consequences of deregulating these mechanisms are poorly understood. Here, we show that disrupting these controls in the budding yeast Saccharomyces(More)
To maintain genomic stability, reinitiation of eukaryotic DNA replication within a single cell cycle is blocked by multiple mechanisms that inactivate or remove replication proteins after G1 phase. Consistent with the prevailing notion that these mechanisms are redundant, we previously showed that simultaneous deregulation of three replication proteins,(More)
The numbers of women of childbearing age in the US with HIV and AIDS from heterosexual transmission continues to rise. Behavioral interventions remain the best means of preventing transmission of HIV. Program planners often implement interventions to promote behavioral change in a wide range of settings such as family planning or sexually transmitted(More)
IN spite of repeated investigations, divergencies still exist as to the nature and relative proportions of the carbohydrates found in the tubers of the Jerusalem artichoke (Helianthus tuberosus). The most detailed account of these carbohydrates has been given by Tanret [1893], who carried out investigations with tubers gathered in September and October.(More)
The Stages of Change (SOC) model was used to implement and evaluate a condom promotion intervention for HIV-positive and at-risk women who were recruited from clinic and community settings in Baltimore and Philadelphia. Participants were assigned to receive standard reproductive health services or enhanced services (standard plus SOC peer advocate(More)
INTRODUCTION In indirect bonding, a white surface layer defect sometimes appears on the custom resin base when brackets are bonded to the stone model with a hydrophilic adhesive. It was surmised that this surface layer might be related to oxygen inhibition. METHODS Scanning electron microscopy and x-ray microanalysis were used to corroborate that this(More)
  • 1