Brett Elmore

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In order to facilitate genetic study of the opportunistic bacterial pathogen Pseudomonas aeruginosa, we isolated a conditional, temperature-sensitive plasmid origin of replication. We mutagenized the popular Pseudomonas stabilizing fragment from pRO1610 in vitro using the Taq thermostable DNA polymerase in a polymerase chain reaction (PCR). Out of(More)
Postoperative pain following thoracic surgery presents a significant challenge, and multiple factors complicate recovery and pain management for this population. Considerable comorbidities often exist in thoracic surgical patients, further limiting therapeutic options. Elements of nociceptive and neuropathic pain may contribute greatly to patient(More)
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