Brendan Wyse

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Chronic infusion of loop diuretics into animals induces structural and functional changes in the distal nephron. These changes include increases in the activity of the thiazide-sensitive Na(+)/Cl(-)-cotransporter (NCC). The NCC was recently demonstrated to be an aldosterone-induced protein. These experiments were designed to test the hypotheses that chronic(More)
All the angiotensin peptides originate from angiotensinogen, a glycoprotein synthesized by several tissues, including the brain and the anterior pituitary. In the rat, immunohistochemistry has been used to localize angiotensinogen in gonadotropes and in uncharacterized cells surrounding sinusoids. Both cell types are capable of secreting angiotensinogen in(More)
A genetically growth hormone (GH)-deficient strain of Lewis rats was used to test the hypothesis that the actions of GH on electrolyte and fluid homeostasis are mediated by the renin-angiotensin-aldosterone system (RAAS). Dwarf rats injected with recombinant bGH (2 x day-1) for 7 days (group GH1+) and 28 days (group GH4+), respectively, were(More)
This study demonstrates the existence of the putative receptor for the hexapeptide (3-8) fragment of angiotensin II (AngIV) on rat astrocytes and neurons grown in cell culture. Binding of 125I-AngIV was saturable and distinct from that of the AngII receptor subtypes. Equilibrium binding was attained in 15 min in astrocytes and 75 min in neurons at 22(More)
A radioimmunoassay (RIA) for the rat growth hormone binding protein (GHBP) was developed using a synthetic peptide (corresponding to the hydrophilic carboxyl-terminal sequence of mouse GHBP) as standard and a monoclonal antibody (MAb 4.3) reactive with this peptide as the primary antibody. The values for GHBP concentration obtained for normal rats using(More)
OpenLearn is a large online educational repository which can be used as a resource for developing and testing Question Generation (QG) systems. OpenLearn is described in detail and its advantages are discussed. To illustrate the use of OpenLearn for QG, a QG system, Ceist , is presented which generates questions from OpenLearn content. Ceist is also offered(More)
The hypothesis, based on previous in vivo data, that angiotensin AT1 receptors are regulated by GH or insulin-like growth factor I (IGF-I) has been investigated in this study using primary cultures of rat astrocytes as a model of AT1 receptor expression. At a dose of 1 ng/ml GH, there was an increase in AT1 density within 4 h and a maximum increase of 361(More)
The First Shared Task Evaluation Challenge on Question Generation took place in 2010 as part of the 3 workshop on Question Generation. The campaign included two tasks: Question Generation from Sentences and Question Generation from Paragraphs. This status report briefly summarizes the motivation, tasks and results. Lessons learned relevant to future(More)
A novel putative receptor for (3-8)angiotensin (AngIV) has recently been found in various tissues, including the brain. However, the localization of AngIV binding sites to specific types of brain cells has yet to be established. In this study tissue culture was used to determine the presence and characteristics of AngIV binding in a glioma cell line (C6)(More)