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Commensal Viruses of Mosquitoes: Host Restriction, Transmission, and Interaction with Arboviral Pathogens
TLDR
It is shown that many ISVs are efficiently transmitted directly from the female mosquito to their progeny via infected eggs, and, moreover, that persistent infection of mosquito cell cultures or whole mosquitoes with ISVs can restrict subsequent infection, replication, and transmission of some mosquito-borne viral pathogens. Expand
The insect-specific Palm Creek virus modulates West Nile virus infection in and transmission by Australian mosquitoes
BackgroundInsect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. These viruses may naturally regulate the transmissionExpand
New genotypes of Liao ning virus (LNV) in Australia exhibit an insect-specific phenotype.
TLDR
High rates of virus detection in progeny reared from infected adult female mosquitoes, coupled with visualization of the virus within the ovarian follicles by immunohistochemistry, suggest that LNV is transmitted transovarially. Expand
A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia.
TLDR
Investigation of the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR and revealed PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Expand
Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia.
TLDR
The detection of a functional furin cleavage motif upstream of the two glycoproteins in the M segment-encoded polyprotein suggests that BADUV may employ a unique strategy to process the virions, and phylogenetically clustered most closely with recently discovered mosquito-borne, insect-specific bunyaviruses in the newly proposed Goukovirus genus. Expand
Discovery and Characterisation of Castlerea Virus, a New Species of Negevirus Isolated in Australia
TLDR
Using a broad-spectrum virus screening assay based on the detection of double-stranded RNA and next-generation sequencing, a novel species of negevirus is detected, from Anopheles, Culex, and Aedes mosquitoes collected in 4 geographically separate regions of Australia. Expand
Viral RNA Intermediates as Targets for Detection and Discovery of Novel and Emerging Mosquito-Borne Viruses
TLDR
Two monoclonal antibodies which show co-localised staining with proteins involved in viral RNA replication in immunofluorescence assay (IFA), suggesting specific reactivity to viral dsRNA are developed and incorporated into a high-throughput, economical ELISA-based screening system for the detection and discovery of viruses from mosquito populations. Expand
A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus but Does Not Replicate in Vertebrate Cells
TLDR
A new virus is detected from mosquito populations of northern Australia that shares genomic sequence similarities to Corriparta virus and did not replicate in several vertebrate cell lines that were permissive to CORV, suggesting that PLV has evolved to have a very restricted host range, indicative of a mosquito-only life cycle. Expand
Infectious DNAs derived from insect-specific flavivirus genomes enable identification of pre- and post-entry host restrictions in vertebrate cells
TLDR
Analysis of these hybrid viruses revealed that ISFs are also restricted from replication in vertebrate cells at the point of entry, and the approaches described here to generate infectious ISF DNAs and chimeric viruses provide unique tools to further dissect the mechanisms of their host restriction. Expand
Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
TLDR
Observations provide additional evidence to support that insect-specific, +ssRNA viruses can be suppressed in cell culture by Wolbachia but -ss RNA viruses may not. Expand
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