Bradley W. Rice

Learn More
In vivo bioluminescence imaging depends on light emitted by luciferases in the body overcoming the effect of tissue attenuation. Understanding this relationship is essential for detection and quantification of signal. We have studied four codon optimized luciferases with different emission spectra, including enzymes from firefly (FLuc), click beetle(More)
In vivo imaging of cells tagged with light-emitting probes, such as firefly luciferase or fluorescent proteins, is a powerful technology that enables a wide range of biological studies in small research animals. Reporters with emission in the red to infrared (>600 nm) are preferred due to the low absorption in tissue at these wavelengths. Modeling of photon(More)
Early detection of tumors can significantly improve the outcome of tumor treatment. One of the most frequently asked questions in cancer imaging is how many cells can be detected non-invasively in a live animal. Although many factors limit such detection, increasing the light emission from cells is one of the most effective ways of overcoming these(More)
It is generally assumed that the specific inhibition of respiratory chain oxidations does not lead to any dissociation of phosphorylation from electron transfer. Support for this view has come from the demonstration that respiration in isolated animal mitochondria can be partially blocked by carbon monoxide (l), low concentrations of azide (2), or antimycin(More)
volume 27 number 7 july 2009 nature biotechnology 708 nm, an extinction coefficient >90,000 M−1 cm−1 and a quantum yield of 0.07. Although the excitation and emission characteristics of IFP1.4 compare favorably to those of existing fluorescent proteins for in vivo imaging, there are a wide range of additional considerations for proteins that are used as in(More)
Therapeutic delivery of human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iCMs) represents a novel clinical approach to regenerate the injured myocardium. However, methods for robust and accurate in vivo monitoring of the iCMs are still lacking. Although superparamagnetic iron oxide nanoparticles (SPIOs) are recognized as a promising tool(More)
Magneto-endosymbionts (MEs) show promise as living magnetic resonance imaging (MRI) contrast agents for in vivo cell tracking. Here we characterize the biomedical imaging properties of ME contrast agents, in vitro and in vivo. By adapting and engineering magnetotactic bacteria to the intracellular niche, we are creating magneto-endosymbionts (MEs) that(More)
The purposes of this study are to characterize magneto-endosymbiont (ME) labeling of mammalian cells and to discern the subcellular fate of these living contrast agents. MEs are novel magnetic resonance imaging (MRI) contrast agents that are being used for cell tracking studies. Understanding the fate of MEs in host cells is valuable for designing in vivo(More)
Impurity-induced turbulence suppression and reduced transport in the DIII-D tokamak* G. R. McKee, M. Murakami, J. A. Boedo, N. H. Brooks, K. H. Burrell, D. R. Ernst, R. J. Fonck, G. L. Jackson, M. Jakubowski, R. J. La Haye, A. M. Messiaen, J. Ongena, C. L. Rettig, B. W. Rice, C. Rost, G. M. Staebler, R. D. Sydora, D. M. Thomas, B. Unterberg, M. R. Wade, and(More)