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AIM To develop a reagent kit that enables multiplex polymerase chain reaction (PCR) amplification of 18 short tandem repeats (STR) and the canine sex-determining Zinc Finger marker. METHODS Validation studies to determine the robustness and reliability in forensic DNA typing of this multiplex assay included sensitivity testing, reproducibility studies,(More)
No geographic differences in mitochondrial DNA (mtDNA) distribution among United States (US) domestic dog populations have been detected to date. To test the hypothesis that regional differences exist, a 608 bp sequence of the canid mtDNA hypervariable region 1 (HV1) from 220 mixed breed animals from the Western, Northeastern, Midwestern, and Southern US(More)
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