Bradley J. Hughes

Learn More
Cardiac lymph from a canine model of myocardial ischemia and reperfusion was examined for evidence of chemotactic activity. Lymph was continuously collected from awake animals before and during a 60-minute coronary artery occlusion and up to 6 hours after the initiation of reperfusion. It was assessed for the ability to activate the following(More)
Indirect immunofluorescent (IIF) techniques employing antitutulin and anticentrosome antibodies were modified for studies of migrating polymorphonuclear (PMNs) leukocytes exposed to gradients or uniform concentrations of chemotactic factor (CF). No significant changes in microtubule (MT) number per cell occurred with chemotactic activation. Significant(More)
Breeding periodicity allows organisms to synchronise breeding attempts with the most favourable ecological conditions under which to raise offspring. For most animal species, ecological conditions vary seasonally and usually impose an annual breeding schedule on their populations; sub-annual breeding schedules will be rare. We use a 16-year dataset of(More)
We describe a rapid, precise, and sensitive radiometric assay for human lactoferrin. In this typical "sandwich"-type assay, anti-human lactoferrin is adsorbed onto a polystyrene sphere and bound lactoferrin is detected by the subsequent binding of 125I-labeled anti-human lactoferrin. The assay is accurate for lactoferrin concentrations of 5 to 1500(More)
To allow a further understanding of the pathogenesis of impaired stimulated locomotion by polymorphonuclear leukocytes (PMNs) in human neonates, we studied cellular orientation by neonatal PMNs in response to well-defined chemotactic gradients (Zigmond orientation chambers) and characterized the cytoplasmic microtubule (MT) complex of neonatal PMNs during(More)
Common Mynas Acridotheres tristis were introduced to the small, isolated barren island of Ascension in the tropical Atlantic Ocean in the 1880s. The founder population of 52 pairs increased at a rate of 2 % per annum. Mynas cause egg losses in other species by puncturing and consuming eggs, puncturing eggs with no consumption or displacing incubating birds(More)
Histologic examination was used to determine the anatomic organization of the feline bulbospongiosus muscle (BSM). Bilaterally symmetrical cranial, middle, and caudal muscle fiber groups were identified. The caudal group was the largest of the 3 groups. Mean cross-sectional diameter of nonfixed BSM fibers was 79 micron. Muscle spindles were not observed in(More)
  • 1