Brad Carlson

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The esterase gene from Streptomyces scabies FL1 was cloned and expressed in Streptomyces lividans on plasmids pIJ486 and pIJ702. In S. lividans, the esterase gene was expressed during later stages of growth and was regulated by zinc, as is seen with S. scabies. The 36-kDa secreted form of the esterase was purified from S. lividans. N-terminal amino acid(More)
Flavopiridol is a novel flavonoid that induces cell cycle arrest at different stages of the cell cycle because of the inhibition of cyclin-dependent kinases (cdks). In previous studies from our laboratory, (B. A. Carlson et al., Cancer Res., 56: 2973–2978, 1996), we observed that exposure of the MCF-7 breast carcinoma cell line to flavopiridol resulted in(More)
Muscarinic receptors (mAChRs) in the pontine reticular formation comprise a critical part of the REM sleep-generating system. Although the role of specific mAChR subtypes remains unclear, data from in vivo microinjection studies suggest that in the pons the M2 subtype is important for REM sleep generation. The present study tested the hypothesis that M2(More)
The gamma-aminobutyric acidA/benzodiazepine receptor contains distinct ligand binding sites for hypnotic barbiturates and benzodiazepines. It is thought that barbiturate-induced sedation is produced, in part, by enhancing agonist binding to this receptor. The present study tested the hypothesis that pentobarbital would enhance benzodiazepine binding in a(More)
Improving nitrogen (N) use efficiency in crop production is important for obvious economic and environmental reasons. Nitrogen fertilization of corn usually provides the greatest return on investment of any crop input; therefore, farmers and their agricultural advisors must insure adequate N is available for the crop to maximize yields and economic returns.(More)
Aurora A kinase (AURKA), a member of the serine/threonine kinase family, plays a critical role in cell division, and it is widely overexpressed in a variety of tumors including glioblastoma (GBM). Alisertib (MLN8237) is an orally administered selective AURKA inhibitor with potent antiproliferative activity, currently undergoing clinical testing in different(More)
The present study used saturation binding analyses to test the hypothesis that pentobarbital would differentially increase the affinity of benzodiazepine binding across brain regions. The results showed that there were significant (p < 0.05) regional differences in the dissociation constant (KD) for tritiated flunitrazepam ([3H]FLU) and that pentobarbital(More)
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