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Microalgae hold great potential as a feedstock for biofuels or bulk protein or treatment of wastewater or flue gas. Realising these applications will require the development of a cost-efficient harvesting technology. Here, we explore the potential of flocculation induced by high pH for harvesting Chlorella vulgaris. Our results demonstrate that flocculation(More)
The isopenicillin-N acyltransferase of Penicillium chrysogenum catalyzes the conversion of the biosynthetic intermediate isopenicillin N to the hydrophobic penicillins. The isopenicillin-N acyltransferase copurified with the acyl-CoA:6-aminopenicillanic acid (6-APA) acyltransferase activity which transfers an acyl residue from acyl-CoA derivatives (e.g.(More)
This paper presents a systematic techno-economical analysis and an environmental impact evaluation of a reverse osmosis (RO) concentrate treatment process using electrodialysis (ED) in view of environmental management of brine discharges. The concentrate originates from a secondary effluent treated by RO. Without any treatment, the concentrate would have to(More)
Cultures of Penicillium chrysogenum, growth with [(35)S]sulfate or labeled amino acids, were examined by ion-exchange chromatography for possible peptidic precursors of penicillin. A sulfur-containing compound, present in both the mycelial extracts and the culture filtrates, was eluted at the location of the synthetic lld-tripeptide(More)
In isolated locations, remote areas, or islands, potable water is precious because of the lack of drinking water treatment facilities and energy supply. Thus, a robust and reliable water treatment system based on natural energy is needed to reuse wastewater or to desalinate groundwater/seawater for provision of drinking water. In this work, a hybrid(More)
Aquatic sediments contaminated with heavy metals originating from mining and metallurgical activities pose significant risk to the environment and human health. These sediments not only act as a sink for heavy metals, but can also constitute a secondary source of heavy metal contamination. A variety of sorbent materials has demonstrated the potential to(More)
Bioleaching of olivine, a natural nickel-containing magnesium-iron-silicate, was conducted by applying chemoorganotrophic bacteria and fungi. The tested fungus, Aspergillus niger, leached substantially more nickel from olivine than the tested bacterium, Paenibacillus mucilaginosus. Aspergillus niger also outperformed two other fungal species: Humicola(More)
The incorporation of valine into the LLD-tripeptide, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine, a precursor of penicillin, was studied by incubating mycelial mats of Penicillium chrysogenum Wis. 49-2105 with double labelled valines. L-valine was incorporated into the LLD-tripeptide without formation of an alpha, beta-didehydrovaline intermediate.(More)
This study aims to evaluate the feasibility of a pellet reactor in reducing the scaling potential in electrodialysis with bipolar membranes for water containing a high concentration of calcium by adding sodium carbonate to precipitate carbonate as calcium carbonate on granular seed material. The optimized operating condition obtained at pH 11.1, and a ratio(More)
Binding of penicillin to the DD-carboxypeptidase of the unstable spheroplast L-form of Proteus mirabilis results in the rapid formation of a modified enzyme-inhibitor complex which in turn undergoes rapid decay into reactivated enzyme and an antibiotically inactive penicillin degradation product. Major antibiotic metabolites recovered from such interactions(More)