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Efficient genome editing in plants using a CRISPR/Cas system
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The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation.
The CRISPR/Cas9 system has been demonstrated to efficiently induce targeted gene editing in a variety of organisms including plants. Recent work showed that CRISPR/Cas9-induced gene mutations inExpand
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Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis
Significance The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been used to generate targeted gene editing in plants. However, it is not knownExpand
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Application of the CRISPR-Cas system for efficient genome engineering in plants.
Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases (ZFNs) (Carroll, 2011), Transcription Activator-Like Effector Nucleases (TALENs) (Mahfouz et al., 2011; Li et al.,Expand
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Defining the mitochondrial stress response in Arabidopsis thaliana.
To obtain a global overview of how mitochondria respond to stress, we aimed to define the plant mitochondrial stress response (MSR). By combining a set of 1196 Arabidopsis thaliana genes thatExpand
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The RCC1 family protein RUG3 is required for splicing of nad2 and complex I biogenesis in mitochondria of Arabidopsis thaliana.
We have identified a mitochondrial protein (RUG3) that is required for accumulation of mitochondrial respiratory chain complex I. RUG3 is related to human REGULATOR OF CHROMOSOME CONDENSATION 1Expand
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Optimized expression of (S)‐carbonyl reductase in Pichia pastoris for efficient production of (S)‐1‐phenyl‐1, 2‐ethanediol
The recombinant (S)‐carbonyl reductase (SCR) in Escherichia coli catalyzed the reduction of 2‐hydroxyacetophenone to (S)‐1‐phenyl‐1,2‐ethanediol (PED) with low efficiency. In this work, its 6×Expand
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[Gene cloning and characterization of a solvent-resistant glucose dehydrogenase from Bacillus sp. YX-1].
OBJECTIVE A gene encoding solvent-resistant glucose dehydrogenase was cloned from Bacillus sp. YX-1 and expressed in Escherichia coli. The recombinant enzyme was then characterized. METHODS TheExpand
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[Expression and subcellular localization of (R)- and (S)-specific carbonyl reductases from Candida parapsilosis in Saccharomyces cerevisia].
OBJECTIVE The (R)- and (S)- specific carbonyl reductases (RCR and SCR) with enhanced green fluorescence protein ( EGFP) were expressed in Saccharomyces cerevisiae W303-1A. By analysis of EGFPExpand
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