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Mutation of beta-glucosidase 2 causes glycolipid storage disease and impaired male fertility.
beta-Glucosidase 2 (GBA2) is a resident enzyme of the endoplasmic reticulum thought to play a role in the metabolism of bile acid-glucose conjugates. To gain insight into the biological function ofExpand
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Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry.
We describe the development of a high performance liquid chromatography mass spectrometry (HPLC-MS) method that allows the identification and quantitation of sterols in mammalian cells and tissues.Expand
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27-Hydroxycholesterol promotes cell-autonomous, ER-positive breast cancer growth.
To date, estrogen is the only known endogenous estrogen receptor (ER) ligand that promotes ER+ breast tumor growth. We report that the cholesterol metabolite 27-hydroxycholesterol (27HC) stimulatesExpand
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Subcellular organelle lipidomics in TLR-4-activated macrophages1[S]
Lipids orchestrate biological processes by acting remotely as signaling molecules or locally as membrane components that modulate protein function. Detailed insight into lipid function requiresExpand
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A Mouse Macrophage Lipidome*♦
We report the lipidomic response of the murine macrophage RAW cell line to Kdo2-lipid A, the active component of an inflammatory lipopolysaccharide functioning as a selective TLR4 agonist andExpand
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Cilia-Associated Oxysterols Activate Smoothened.
Primary cilia are required for Smoothened to transduce vertebrate Hedgehog signals, but how Smoothened accumulates in cilia and is activated is incompletely understood. Here, we identifyExpand
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Liver-Derived Signals Sequentially Reprogram Myeloid Enhancers to Initiate and Maintain Kupffer Cell Identity.
Tissue environment plays a powerful role in establishing and maintaining the distinct phenotypes of resident macrophages, but the underlying molecular mechanisms remain poorly understood. Here, weExpand
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Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi
The oncoprotein Smoothened (SMO), a G-protein-coupled receptor (GPCR) of the Frizzled-class (class-F), transduces the Hedgehog signal from the tumour suppressor Patched-1 (PTCH1) to theExpand
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Bazedoxifene and conjugated estrogen prevent diet-induced obesity, hepatic steatosis, and type 2 diabetes in mice without impacting the reproductive tract.
Despite the capacity of estrogens to favorably regulate body composition and glucose homeostasis, their use to combat obesity and type 2 diabetes is not feasible, because they promote sexExpand
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Oxysterols provide innate immunity to bacterial infection by mobilizing cell surface accessible cholesterol
Cholesterol 25-hydroxylase ( CH25H ) is an interferon-stimulated gene that converts cholesterol to the oxysterol 25-hydroxycholesterol (25HC). Circulating 25HC modulates essential immunologicalExpand
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