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Mechanistic analysis of the pump cycle of the KdpFABC P-type ATPase.
Various mechanistic pump cycle models were derived from the general Post-Albers scheme of P-type ATPases and discussed in the framework of the experimental evidence to propose a possible molecular pump cycle for KdpFABC. Expand
Role of protons in the pump cycle of KdpFABC investigated by time-resolved kinetic experiments.
The time-resolved kinetics of the KdpFABC complex solubilized in Aminoxide WS-35 was investigated by ATP concentration jump experiments and pH dependence experiments revealed different roles of H(+) in the transport mechanism. Expand
Laser desorption and ionization time-of-flightversus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of and metal complexes.
The matrix-free approach was tested for the analysis of transition metal complexes and the detection limit in the MALDI-TOF MS analysis of [PtCl 2(dach)] and [RuCl2(bipy)2]Cl complexes was lower than that determined in LDI-toF MS. Expand
Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A2.
Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activityExpand
KdpFABC reconstituted in Escherichia coli lipid vesicles: substrate dependence of the transport rate.
The results indicate that high cytoplasmic K(+) concentrations have an inhibitory effect on the KdpFABC complex, and ion pumping was activated by addition of ATP to the external medium which corresponds to the cy toplasm under physiological conditions. Expand
Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)2Cl2]Cl acquired with different matrices
In this work, the matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectra of two cationic complexes, i.e., [PdCl(dien)]Cl and [Ru(en)2Cl2]Cl, acquired underExpand
Assaying P-Type ATPases Reconstituted in Liposomes.
Two reconstitution methods by dialysis and the use of potential-sensitive fluorescence dyes to study transport properties of two P-type ATPases, the Na,K-ATPase from rabbit kidney and the K(+)-transporting KdpFABC complex from E. coli are described. Expand