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Putrescine, spermidine, and spermine levels were measured in the cortex, caudoputamen, and hippocampus of rats during 30 min of severe forebrain ischemia (induced by occlusion of both carotid and vertebral arteries) and subsequent recirculation. During ischemia, polyamine levels did not change significantly. During postischemic recirculation, however,(More)
Depression is frequently encountered in Parkinson's disease and was seen to occur in 14 of 26 patients studied. The levels of 5-hydroxyindoleacetic acid (5-HIAA), the main metabolite of serotonin (5-HT), in CSF samples of the patients were significantly lower than in those of controls. However, within the group of patients the levels of 5-HIAA in CSF(More)
To elucidate whether the high sensitivity of gerbil compared with rat hippocampus to metabolic stress results from tissue-specific or hemodynamic factors, ischemia-induced metabolic disturbances [energy metabolism and protein synthesis rate (PSR)] were studied using the in vitro model of the hippocampal slice preparation. At the end of in vitro ischemia,(More)
Free choline and ATP contents were measured in Mongolian gerbil hippocampal slices (tissue) and incubation media (media) during exposure to 30 min of aglycemia, high potassium, anoxia, or ischemia. Changes in choline levels reflected the degree of energy reduction, lower ATP levels being associated with high choline (4-fold increase during exposure to high(More)
A technique for the vascular perfusion of the guinea pig head in vivo, suitable for measurements of blood-to-brain transport under controlled conditions of arterial inflow, has been developed. With a perfusion pressure ranging between 13 and 18 kPa and PCO2 in the arterial inflow of 5 and 5.5 kPa, cerebral blood flow, measured with [14C]butanol, was about 1(More)
The extent of RNA editing of the glutamate receptor subunit GluR5 mRNA was evaluated in the cortex, hippocampus and cerebellum of embryonic rat brains, aged 19 days, and adult brains, aged 2-3 months. RNA was isolated and transcribed into cDNA, which was used as template for amplifying a PCR product across the edited region of the GluR5 subunit.(More)
Protein synthesis was measured in hippocampal slices which were exposed to glutamate (1 mM or 10 mM) or which were deprived of glucose and oxygen (‘in vitro ischemia’) for 15 min. Glutamate at 1 mM, a concentration estimated to occur duringin vivo ischemia did not affect protein synthesis. Ten mM glutamate inhibited protein synthesis immediately after(More)
In Mongolian gerbils (Meriones unguiculatus) cerebral ischemia was produced by occlusion of the right common and the left external carotid arteries. Gerbils were classified according to their neurological appearance as "symptom-negative" (8 animals), "mild symptoms" (unilateral hemiparesis, 10 animals) and "severe symptoms" (hemiparesis and rolling(More)
The concentration of prostaglandin F2 alpha (PGF2 alpha) was measured in cerebrospinal fluid (CSF) of 50 patients with recent stroke divided into three groups in respect to the time (up to 24 h) that expired between cerebrovascular insult and CSF sampling. 10 of the additionally studied persons were neurologically normal (control group). None of the(More)
The post-ischemic effects on cerebral cortex and basal ganglia monoamine levels and monoamine oxidase (MAO A and B) and catechol-O-methyl transferase (COMT) activities were evaluated in Mongolian gerbils (Meriones unguiculatus) subjected to bilateral common carotid arteries occlusion for 15 min and reflow for 7 days. Disorders of monoamine metabolism was(More)