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agr-Mediated Dispersal of Staphylococcus aureus Biofilms
TLDR
It is reported that repression of agr is necessary to form a biofilm and that reactivation of agR in established biofilms through AIP addition or glucose depletion triggers detachment, and that the dispersal mechanism requires extracellular protease activity. Expand
Self-generated diversity produces "insurance effects" in biofilm communities.
TLDR
It is shown that the opportunistic pathogen Pseudomonas aeruginosa undergoes extensive genetic diversification during short-term growth in biofilm communities, suggesting that self-generated diversity in biofilms provides a form of biological insurance that can safeguard the community in the face of adverse conditions. Expand
Identification of Genes Involved in Polysaccharide-Independent Staphylococcus aureus Biofilm Formation
TLDR
A screen for biofilm defective mutants identified novel loci involved in S. aureus biofilm formation and underscored the importance of extracellular protease activity and autolysis in biofilm development. Expand
Modulation of eDNA Release and Degradation Affects Staphylococcus aureus Biofilm Maturation
TLDR
A model in which the opposing activities of the cid and lrg gene products control cell lysis and genomic DNA release during biofilm development, while staphylococcal thermonuclease functions to degrade the eDNA, possibly as a means to promote biofilm dispersal is suggested. Expand
Endogenous oxidative stress produces diversity and adaptability in biofilm communities
TLDR
It is hypothesized that the mutations produced could promote the adaptation of biofilm communities to changing conditions in addition to generating diversity, and it was found that the oxidative stress-break repair mechanism increased the emergence of antibiotic-resistant bacteria. Expand
Rhamnolipids mediate detachment of Pseudomonas aeruginosa from biofilms
TLDR
It is shown that Pseudomonas aeruginosa variants that exhibit accelerated biofilm detachment are mediated by a mechanism requiring the biosurfactant rhamnolipid and that this detachment mechanism rapidly restores antibiotic sensitivity to separating bacteria. Expand
Functional Amyloids Composed of Phenol Soluble Modulins Stabilize Staphylococcus aureus Biofilms
TLDR
The discovery of a novel extracellular fibril structure that promotes S. aureus biofilm integrity is reported, and data is presented showing that soluble PSM peptides disperse biofilms while polymerized peptides do not. Expand
Interconnections between Sigma B, agr, and Proteolytic Activity in Staphylococcus aureus Biofilm Maturation
TLDR
These findings indicate that the lack of SigB activity results in increased RNAIII expression, thus elevating extracellular protease levels and altering the murein hydrolase activity profile. Expand
Fluorescent reporters for Staphylococcus aureus.
TLDR
A toolbox of fluorescent reporter plasmids will facilitate cell labeling for a variety of different experimental applications, and a constitutive YFP reporter displayed stable, robust labeling of biofilm growth in a flow-cell apparatus. Expand
Staphylococcal biofilm disassembly.
TLDR
New developments in staphylococcal biofilm disassembly are reviewed and set them in the context of potential strategies to control biofilm infections. Expand
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