Birgit Rengstl

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Photosystem II (PSII) is an integral-membrane, multisubunit complex that initiates electron flow in oxygenic photosynthesis. The biogenesis of this complex machine involves the concerted assembly of at least 20 different polypeptides as well as the incorporation of a variety of inorganic and organic cofactors. Many factors have recently been identified that(More)
Early steps in the biogenesis of Photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803 are thought to occur in a specialized membrane fraction that is characterized by the specific accumulation of the PSII assembly factor PratA and its interaction partner pD1, the precursor of the D1 protein of PSII. Here, we report the molecular(More)
Photosystem II (PSII) is a multiprotein complex that functions as a light-driven water:plastoquinone oxidoreductase in photosynthesis. Assembly of PSII proceeds through a number of distinct intermediate states and requires auxiliary proteins. The photosynthesis affected mutant 68 (pam68) of Arabidopsis thaliana displays drastically altered chlorophyll(More)
In the cyanobacterium Synechocystis sp PCC 6803, early steps in thylakoid membrane (TM) biogenesis are considered to take place in specialized membrane fractions resembling an interface between the plasma membrane (PM) and TM. This region (the PratA-defined membrane) is defined by the presence of the photosystem II (PSII) assembly factor PratA (for(More)
In vascular plants, the chloroplast NAD(P)H dehydrogenase complex (NDH-C) is assembled from five distinct subcomplexes, the membrane-spanning (subM) and the luminal (subL) subcomplexes, as well as subA, subB, and subE. The assembly process itself is poorly understood. Vascular plant genomes code for two related intrinsic thylakoid proteins,(More)
The de novo assembly of photosystem II (PSII) depends on a variety of assisting factors. We have previously shown that two of them, namely, YCF48 and Sll0933, mutually interact and form a complex (Rengstl et al. in J Biol Chem 286:21944–21951, 2011). To gain further insights into the importance of the YCF48/Sll0933 interaction, an ycf48 − sll0933 − double(More)
The assembly and repair of photosystem II (PSII) is facilitated by a variety of assembly factors. Among those, the tetratricopeptide repeat (TPR) protein Slr0151 from Synechocystis sp. PCC 6803 (hereafter Synechocystis) has previously been assigned a repair function under high light conditions (Yang et al., 2014). Here, we show that inactivation of slr0151(More)
Photosynthesis occurs in thylakoids, a highly specialized membrane system. In the cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803), the thylakoids are arranged parallel to the plasma membrane and occasionally converge towards it to form biogenesis centers. The initial steps in photosystem II (PSII) assembly are thought to take place(More)
Anna Stengel,a Irene L. Gügel,b Daniel Hilger,c Birgit Rengstl,a Heinrich Jung,c and Jörg Nickelsena,1 aMolekulare Pflanzenwissenschaften, Biozentrum Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany b Biochemie und Physiologie der Pflanzen, Biozentrum Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany(More)
Current molecular analyses suggest that initial steps of the biogenesis of cyanobacterial photosystems progress in a membrane subfraction representing a biosynthetic center with contact to both plasma and thylakoid membranes. This special membrane fraction is defined by the presence of the photosystem II assembly factor PratA. The proposed model suggests(More)