Birgit Arnholdt-Schmitt

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Abbreviations: 2,4-D: 2,4-dichlorophenoxyacetic acid ABA: abscisic acid ABI3: ABI gene family member 3 ANP1: Arabidopsis NPK1-like protein kinase AP: ascorbate peroxidase AOX: alternative oxidase DNA: deoxyribonucleic acid Fe-EDTA: ferric ethylenediaminetetraacetic acid M: molar concentration MAPK: mitogen-activated protein kinase NO: Nitric oxide PGRs:(More)
Alternative oxidase (AOX) has been proposed as a functional marker candidate in a number of events involving cell differentiation, including rooting efficiency in semi-hardwood shoot cuttings of olive (Olea europaea L.). To ascertain the general importance of AOX in olive rooting, the auxin-induced rooting process was studied in an in vitro system for(More)
Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization. Hypericum perforatum L. (St. John's wort) is a field growing plant that is frequently exposed to a variety of(More)
Plant alternative oxidase (AOX) is a mitochondrial inner membrane enzyme involved in alternative respiration. The critical importance of the enzyme during acclimation upon stress of plant cells is not fully understood and is still an issue of intensive research and discussion. Recently, a role of AOX was suggested for the ability of plant cells to change(More)
'Genomic design' refers to the structural organization of gene sequences. Recently, the role of intron sequences for gene regulation is being better understood. Further, introns possess high rates of polymorphism that are considered as the major source for speciation. In molecular breeding, the length of gene-specific introns is recognized as a tool to(More)
Calorespirometric measurements proved to be useful for phenotyping temperature response in terms of optimum temperatures for growth and low temperature limits for growth respiration in diverse carrot genotypes. High and low-temperature tolerance is an important trait in many breeding programs, but to date, improvement strategies have had limited success.(More)
Stress-adaptive cell plasticity in target tissues and cells for plant biomass growth is important for yield stability. In vitro systems with reproducible cell plasticity can help to identify relevant metabolic and molecular events during early cell reprogramming. In carrot, regulation of the central root meristem is a critical target for yield-determining(More)
We propose a novel concept and tool kit for predictive phenotyping. The proposed technology measures respiration properties as functions of growth conditions to identify genotypes with higher plasticity via homeostasis and adaptive morphophysiology. Combining calorespirometry, oxygen isotope analysis and functional-marker-assisted selection ('CalOxy-FMAS')(More)
Incorrectly or simply not annotated data is largely increasing in most public databases, undoubtedly caused by the rise in sequence data and the more recent boom of genomic projects. Molecular biologists and bioinformaticists should join efforts to tackle this issue. Practical challenges have been experienced when studying the alternative oxidase (AOX) gene(More)