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Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA
Evidence for a possible route for DNA demethylation in animals is suggested. The prevalent DNA modification in higher organisms is the methylation of cytosine to 5-methylcytosine (5mC), which isExpand
Synergistic Function of DNA Methyltransferases Dnmt3a and Dnmt3b in the Methylation of Oct4 and Nanog
ABSTRACT DNA methylation plays an important role in gene silencing in mammals. Two de novo methyltransferases, Dnmt3a and Dnmt3b, are required for the establishment of genomic methylation patterns inExpand
Histone tails regulate DNA methylation by allosterically activating de novo methyltransferase
Cytosine methylation of genomic DNA controls gene expression and maintains genome stability. How a specific DNA sequence is targeted for methylation by a methyltransferase is largely unknown. Here,Expand
Gadd45a promotes DNA demethylation through TDG
Growth arrest and DNA-damage-inducible protein 45 (Gadd45) family members have been implicated in DNA demethylation in vertebrates. However, it remained unclear how they contribute to theExpand
The Swr1 chromatin-remodeling complex prevents genome instability induced by replication fork progression defects
Genome instability is associated with tumorigenesis. Here, we identify a role for the histone Htz1, which is deposited by the Swr1 chromatin-remodeling complex (SWR-C), in preventing genomeExpand
Identification of Exo1-Msh2 interaction motifs in DNA mismatch repair and new Msh2-binding partners
Eukaryotic DNA mismatch repair (MMR) involves both exonuclease 1 (Exo1)-dependent and Exo1-independent pathways. We found that the unstructured C-terminal domain of Saccharomyces cerevisiae Exo1Expand
Cdc73 suppresses genome instability by mediating telomere homeostasis
Defects in the genes encoding the Paf1 complex can cause increased genome instability. Loss of Paf1, Cdc73, and Ctr9, but not Rtf1 or Leo1, caused increased accumulation of gross chromosomalExpand
SUMO E3 ligase Mms21 prevents spontaneous DNA damage induced genome rearrangements
Mms21, a subunit of the Smc5/6 complex, possesses an E3 ligase activity for the Small Ubiquitin-like MOdifier (SUMO). Here we show that the mms21-CH mutation, which inactivates Mms21 ligase activity,Expand
Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
Significance By performing a targeted genetic screen of temperature-sensitive mutations, this study identified 94 essential Saccharomyces cerevisiae genome instability suppressing (eGIS) genes and 38Expand
Mechanisms underlying genome instability mediated by formation of foldback inversions in Saccharomyces cerevisiae
Foldback inversions, also called inverted duplications, have been observed in human genetic diseases and cancers. Here, we used a Saccharomyces cerevisiae genetic system that generates grossExpand