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OBJECTIVE In order to clarify the trend of change in lactase and its gene regulation pattern, the authors determined the lactase activities and mRNA levels in BALB/c mice at different ages. METHODS The lactase activities and mRNA levels of the 3-, 5-, 7-, 9- week old mice were determined by the modified method of Dahlqvist and in situ hybridization(More)
UT-B is a urea transporter protein expressed in the kidney and in many non-renal tissues including erythrocytes, brain, heart, bladder and the testis. The objective of this study was to determine the phenotype of UT-B deletion in the heart. UT-B expression in the heart was studied in wild-type mice vs UT-B null mice by utilizing RT-PCR and Western blot. A(More)
OBJECTIVE To study the morphological features of secundum atrial septal defect (ASD) in adult and the implications for transcatheter closure. METHODS Transcatheter closure using Amplatzer duct occluder was performed in 272 adult patients with ASD from September 1997 to December 2005. The morphological features were evaluated by transthoracic(More)
OBJECTIVE To explore the role of altered characteristics of intestinal dendritic cell (DC) in the induction of visceral hyperalgesia through the activation of mast cells in a rat model of irritable bowel syndrome (IBS). METHODS A total of 20 Sprague-Dawley rats were divided into modeling and control groups (n = 10 each). The IBS rat model was established(More)
OBJECTIVE To observe the expression of protein arginine N-methyltransferase (PRMT) genes in the lung and spleen of E3 rats with acute asthma. METHODS E3 rats with ovalbumin-induced pulmonary inflammation were divided into two groups (n=10), and the validity of the acute asthma model was evaluated by histological observation with HE and PAS staining and by(More)
OBJECTIVE (99)Tc(m) labeled C2A domain of synaptotagmin I ((99)Tc(m)-Syt I-C2A) is used for noninvasive detection of vulnerable atherosclerotic plaque. METHODS Recombinant C2A domain of synaptotagmin I, overexpressed in E. Coli, was thiolated with 2-iminothiolane (2-IT) and labeled with (99)Tc(m). Atherosclerotic plaques were produced in 5 rabbits by(More)
GNAO1 (guanine nucleotide-binding protein, α-activating activity polypeptide O) is a member of the subunit family of Gα proteins, which are molecular switchers controlling signal transductions and whose deregulation can promote oncogenesis. HCC (hepatocellular carcinoma) is one of the malignant tumours around the world, which summons novel biomarkers or(More)
OBJECTIVE To investigate the relationship between the single nucleotide polymorphisms (SNP) of tumor necrosis factor-alpha (TNF-alpha) promoter genes and susceptibility to gastric adenocarcinoma or gastric adenocarcinoma with helicobacter pylori (Hp) infection. METHODS The SNPs of the TNF-alpha (-238G/A and -308G/A) were determined by gene chip in 130(More)
OBJECTIVE To study the epidemiologic features of functional bowel disorders (FBD) among medical students and science and engineering students in Zhejiang province, China, and to explore the relationship between psychological factors and FBD. METHODS This study was a survey of random sampling based on the Rome III Diagnostic Questionnaire (Rome III-DQ)(More)