Bihari L. Gupta

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The erythrocytes from control (C), diabetic (D) and insulin-treated diabetic (D+I) rats were separated into three ageing groups (TAG) i.e., light dense (young cells), intermediate-dense (middle-aged cells) and heavy-dense (old aged cells) samples. The activities of enzymes and metabolites changed from young to old cells in the following manner: (1) Increase(More)
Insulin receptor activity and its relationship with catecholamines in rat young, middle aged and old red blood cells were investigated in experimental hypothyroidism and hyperthyroidism. In control animals, a loss of insulin receptor activity was found with cellular ageing and increased levels of norepinephrine, epinephrine and glycosylated hemoglobin.(More)
The activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and protein levels were measured in vitro incubation of tissue homogenate of brain and gonads with testosterone propionate (TP) in Channa punctatus (teleost). The activities of LDH and MDH were found to be highest in the ovarian tissues in comparison to the testis and the brain in(More)
Experimentally induced diabetic rats were treated separately with insulin and vanadate. The activities of hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6PDH) were increased in reticulocyte hemolysate isolated from the diabetic rats and were restored to normal levels by insulin. The restoration was not detected in vanadate treated diabetic(More)
Hexokinase and glucose-6-phosphate dehydrogenase were assayed in various circulating age fractions i.e., young, middle-aged and old red cell from control, diabetic and insulin-treated diabetic rats. An increase in the activity of hexokinase was observed in three age-wise separated fractions of red cells from diabetic animals in comparison to control. The(More)
Vanadium compounds are potent in controlling elevated blood glucose levels in experimentally induced diabetes. However the toxicity associated with vanadium limits its role as therapeutic agent for diabetic treatment. A vanadium compound sodium orthovanadate (SOV) was given to alloxan-induced diabetic Wistar rats in lower doses in combination withTrigonella(More)
The reticulocytes and the ageing red blood cells (RBCs) namely young (Y), middle-aged (M) and old RBCs (O) of female Wistar rats from different groups such as control animals (C), controls treated with vanadate (C + V), alloxan-induced diabetic (D), diabetic-treated with insulin (D + I) and vanadate (D + V), were fractionated on a percoll/BSA gradient. The(More)
The erythrocytes from control (C), diabetic (D) and thyroxine-treated diabetic (D+T4) rats were separated into three ageing groups (TAG) like light dense (young cells), intermediate-dense (middle-aged cells) and heavy-dense (old aged cells). Thyroxine reversed the diabetic defense enzymes to normal values like catalase in young cells,(More)
The levels of catalase (CAT), glutathione-s-transferase (GST), reduced glutathione (GSH) and oxidised glutathione (GSSG) were measured in red blood cells from control (C) and diabetic rats (D). Diabetes was induced by alloxan administration and diabetic rats were treated with insulin (D+I) and thyroxine (D+T4). On the third day of insulin withdrawal the CAT(More)