Bianca Sclavi

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Focusing on the DNA-bridging nucleoid proteins Fis and H-NS, and integrating several independent experimental and bioinformatic data sources, we investigate the links between chromosomal spatial organization and global transcriptional regulation. By means of a novel multi-scale spatial aggregation analysis, we uncover the existence of contiguous clusters of(More)
In most bacteria, the timing and synchrony of initiation of chromosomal replication are determined by the binding of the AAA(+) protein DnaA to a set of high- and low-affinity sites found within the origin of chromosomal replication (oriC). Despite the large amount of information on the role and regulation of DnaA, the actual structure of the DnaA-oriC(More)
BACKGROUND In Escherichia coli, overlapping rounds of DNA replication allow the bacteria to double in faster times than the time required to copy the genome. The precise timing of initiation of DNA replication is determined by a regulatory circuit that depends on the binding of a critical number of ATP-bound DnaA proteins at the origin of replication,(More)
Growing evidence suggests that many vertebrate lineages are evolving at significantly different rates. As a first approximation of evolutionary rates, we assessed the amount of neutral (dS) and non-neutral (dN) substitutions that have accumulated within and across sister clades since the time of their divergence. We found that in fish, tetraodontiformes(More)
The H-NS chromosome-organizing protein in E. coli can stabilize genomic DNA loops, and form oligomeric structures connected to repression of gene expression. Motivated by the link between chromosome organization, protein binding and gene expression, we analyzed publicly available genomic data sets of various origins, from genome-wide protein binding(More)
(2015) The nucleoid as a smart polymer. Science has a close but very complex relationship with technology (Latour, 1987). A simple phenomenon is that technology enables science by offering tools that provide new data or new kinds of data. In other cases, aspects or views of the empirical world may remain invisible until technology builds something that(More)
Salamanders (urodela) have among the largest vertebrate genomes, ranging in size from 10 to over 80 pg. The urodela are divided into ten extant families each with a characteristic range in genome size. Although changes in genome size often occur randomly and in the absence of selection pressure, non‐random patterns of genome size variation are evident among(More)
Very low levels of genetic diversity have been reported in vertebrates with large genomes, notably salamanders and lungfish [1-3]. Interpreting differences in heterozygosity, which reflects genetic diversity in a population, is complicated because levels of heterozygosity vary widely between conspecific populations, and correlate with many different(More)
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