Bhumi Nath Tripathi

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To explore the adaptability of bread wheat to dehydration stress, we screened 28 cultivars collected from different agroclimatic zones, on the basis of malonaldehyde content as biochemical marker in roots of wheat seedlings during germination and classified them as highly tolerant, tolerant, sensitive and highly sensitive. From this primary screening, ten(More)
BACKGROUND AND AIMS The 2-Cys peroxiredoxin (Prx) A protein of Arabidopsis thaliana performs the dual functions of a peroxidase and a molecular chaperone depending on its conformation and the metabolic conditions. However, the precise mechanism responsible for the functional switching of 2-Cys Prx A is poorly known. This study examines various(More)
Peroxiredoxins (Prx) have received considerable attention during recent years. This study demonstrates that two typical Pseudomonas-derived 2-Cys Prx proteins, PpPrx and PaPrx can alternatively function as a peroxidase and chaperone. The amino acid sequences of these two Prx proteins exhibit 93% homology, but PpPrx possesses an additional cysteine residue,(More)
Osmotic stress induced by dehydration and salinity, is among the major abiotic stresses that adversely impacts crop productivity and plants often display cultivar-dependent response against osmotic imbalance. To better understand the molecular mechanisms underlying differential responses to dehydration, transcriptome changes of two contrasting wheat(More)
Alkyl hydroperoxide reductase subunit C from Pseudomonas aeruginosa PAO1 (PaAhpC) is a member of the 2-Cys peroxiredoxin family. Here, we examined the peroxidase and molecular chaperone functions of PaAhpC using a site-directed mutagenesis approach by substitution of Ser and Thr residues with Cys at positions 78 and 105 located between two catalytic(More)
Eun Mi Lee1,y, Seung Sik Lee1,y, Bhumi Nath Tripathi, Hyun Suk Jung, Guang Ping Cao, Yuno Lee, Sudhir Singh, Sung Hyun Hong, Keun Woo Lee, Sang Yeol Lee, Jae-Young Cho and Byung Yeoup Chung* Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup 580-185, Republic of Korea, Department of Biochemistry, College(More)
Eun Mi Lee1,y, Seung Sik Lee1,y, Bhumi Nath Tripathi, Hyun Suk Jung, Guang Ping Cao, Yuno Lee, Sudhir Singh, Sung Hyun Hong, Keun Woo Lee, Sang Yeol Lee, Jae-Young Cho and Byung Yeoup Chung* Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup 580-185, Republic of Korea, Department of Biochemistry, College(More)
In this work, the role of cation-pi and CH...pi interactions in the activity of glutaredoxins is analysed. Among the proteins, an average of 1 cation-pi interaction per 109 residues and an average of 1 CH...pi interaction per 16 residues were found. These interactions were influenced by long-range contacts whereas short- and medium-range contacts were found(More)
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