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Journals and Conferences
The Autographa californica multinucleocapsid polyhedrosis virus homologous region sequence hr1 enhances transcription from the viral polyhedrin promoter in Spodoptera frugiperda insect cells and independently functions as an origin of replication (ori) sequence. The binding of the host nuclear protein, hr1-binding protein (hr1-BP), is crucial for the… (More)
Mung bean seedlings contain three isoenzymes of superoxide dismutase. The variations in superoxide dismutase activity and isoenzyme pattern in the whole seedling and in different parts of the seedling such as embryonic axis, cotyledons, roots, and leaves is reported. Superoxide dismutase-I and II are localized in the chloroplasts.
Three isoenzymes of superoxide dismutase were identified in germinating seeds of Pennisetum typhoideum. Isoenzyme-I was localized in chloroplasts and isoenzyme-II was found to be associated with mitochondria. By using inhibitors isoenzyme-I and III were tentatively identified as Cu-Zn superoxide dismutases and isoenzyme-II as a Mn-containing enzyme.
The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable of functioning as an enhancer of… (More)
Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cells of BALB/c mouse in vivo. The membrane… (More)
The baculovirus expression vector system has emerged as the system of choice for the expression of a number of heterologous genes of both prokaryotic and eukaryotic origin. This system utilizes the baculovirus very late, hyperactive polyhedrin and p10 promoters to drive the transcription of foreign genes. Regulation of transcription from these promoters is… (More)
The baculovirus system is an extremely powerful tool for expression of heterologous genes in eukaryotic environment. A multiple expression vector, pBacUCmP3, was constructed which harbored two copies of the Autographa californica nuclear polyhedrosis virus very late gene promoter and the Drosophila melanogaster 70-kDa heat-shock protein (hsp70) promoter… (More)