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Fluorescent Proteins as Genetically Encoded FRET Biosensors in Life Sciences
TLDR
Fluorescence- or Förster resonance energy transfer (FRET) is a measurable physical energy transfer phenomenon between appropriate chromophores, when they are in sufficient proximity, usually within 10 nm. Expand
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Fluorescence colocalization microscopy analysis can be improved by combining object‐recognition with pixel‐intensity‐correlation
The question whether two proteins interact with each other or whether a protein localizes to a certain region of the cell is often addressed with fluorescence microscopy and analysis of a potentialExpand
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Sequence-function correlations and dynamics of ERG isoforms. ERG8 is the black sheep of the family
The transcription factor ERG is known to have divergent roles. On one hand, it acts as differentiation factor of endothelial cells. On the other hand, it has pathological roles in various cancers.Expand
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Rare Human Missense Variants can affect the Function of Disease-Relevant Proteins by Loss and Gain of Peroxisomal Targeting Motifs
Single nucleotide variants (SNVs) resulting in amino acid substitutions (i.e., missense variants) can affect protein localization by changing or creating new targeting signals. Here, we studied theExpand
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Novel approach for accurate tissue-based protein colocalization and proximity microscopy
Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneousExpand
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Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells
FRET (Fluorescence Resonance Energy Transfer) measurements are commonly applied to proof protein-protein interactions. However, standard methods of live cell FRET microscopy and signal normalizationExpand
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A Novel FRET Approach Quantifies the Interaction Strength of Peroxisomal Targeting Signals and Their Receptor in Living Cells.
Measuring Förster-resonance-energy-transfer (FRET) efficiency allows the investigation of protein-protein interactions (PPI), but extracting quantitative measures of affinity necessitates highlyExpand
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Ikk2-mediated inflammatory activation of arterial endothelial cells promotes the development and progression of atherosclerosis.
BACKGROUND AND AIMS Inflammatory activation of endothelial cells is considered to be the first step in the development of atherosclerosis. Here, we determined the consequences of chronic endothelialExpand
Addendum: Hochreiter, B.; Pardo-Garcia, A.; Schmid, J.A. Fluorescent Proteins as Genetically Encoded FRET Biosensors in Life Sciences. Sensors 2015, 15, 26281–26314
TLDR
The authors wish to add an Acknowledgments section to their paper published in Sensors [1], doi:10.3390/s151026281, website: https://www.mdpi.com/1424-8220/. Expand