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The endoplasmic reticulum (ER) in animal cells is an extensive, morphologically continuous network of membrane tubules and flattened cisternae. The ER is a multifunctional organelle; the synthesis of membrane lipids, membrane and secretory proteins, and the regulation of intracellular calcium are prominent among its array of functions. Many of these(More)
The composition of dark-adapted and illuminated retinal rod outer and inner segments and mitochondria was determined with electron probe X-ray micro-analysis of cryosections. The concentration of Ca in the outer segment was 0.4 mmol/kg dry wt. (0.1 Ca/rhodopsin) and did not measurably change upon illumination with saturating light for 5 min. The(More)
  • B Walz
  • 1992
Deeply dark adapted (1 h) photoreceptor cells of the honey bee drone show a light-induced enhancement of sensitivity (facilitation) as an aftereffect of illumination or in the presence of dim backgrounds. The Ca(2+)-dependency of this effect was studied: Reduction of extracellular Ca2+ to 0.1 mM decreases the sensitivity of a dark adapted cell, and the(More)
1. Blowfly salivary glands have been used extensively as a model system for the analysis of inositol phosphate-dependent signal transduction. To detect and characterize changes in intracellular free calcium ([Ca2+]i) that might be expected to be triggered by stimulation with serotonin (5-HT), we have carried out digital calcium-imaging experiments on intact(More)
Intercellular Ca2+ signaling in intact salivary glands of the blowfly Calliphora erythrocephala was studied by fluorimetric digital imaging combined with microinjection of putative messenger molecules. Iontophoretic injection of D-myo-inositol 1,4, 5-trisphosphate (InsP3) into salivary gland cells evoked regenerative intercellular Ca2+ waves that spread(More)
Light-dependent changes in the positioning of organelles in photoreceptor cells of arthropods are a well-known phenomenon. In this study, we examine the role of the cytoskeleton in these light-dependent antagonistic movements. In dark-adapted photoreceptor cells of the locust Schistocerca gregaria, prominent sacs of smooth endoplasmic reticulum (ER) oppose(More)
The leech photoreceptor forms a unicellular epithelium: every cell surrounds an extracellular "vacuole" that is connected to the remaining extracellular space via narrow clefts containing pleated septate junctions. We analyzed the complete structural layout of all septa within the junctional complex in elastic brightfield stereo electron micrographs of(More)
Invertebrate microvillar photoreceptors contain an extensive, morphologically continuous endoplasmic reticulum (ER) that comprises several distinct subregions. Most prominent is the smooth submicrovillar ER, a sponge-like cisternal network underneath the photoreceptive microvillar membrane. The submicrovillar ER spatially separates the microvilli and a(More)
Light stimulation of invertebrate microvillar photoreceptors causes a large rapid elevation in Cai, shown previously to modulate the adaptational state of the cells. Cai rises, at least in part, as a result of Ins(1,4,5)P3-induced Ca2+ release from the submicrovillar endoplasmic reticulum (ER). Here, we provide evidence for Ca(2+)-induced Ca2+ release(More)
The acinar salivary glands of cockroaches receive a dual innervation from the subesophageal ganglion and the stomatogastric nervous system. Acinar cells are surrounded by a plexus of dopaminergic and serotonergic varicose fibers. In addition, serotonergic terminals lie deep in the extracellular spaces between acinar cells. Excitation-secretion coupling in(More)