Bernd Stieger

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In hepatocytes, all newly synthesized plasma membrane (PM) proteins so far studied arrive first at the basolateral domain; apically destined proteins are subsequently endocytosed and sorted to the apical domain via transcytosis. A mechanism for the sorting of newly synthesized glycophosphatidylinositol (GPI)-linked proteins has been proposed whereby they(More)
We have used pulse-chase metabolic radiolabeling with L-[35S]methionine in conjunction with subcellular fractionation and specific protein immunoprecipitation techniques to compare the posttranslational transport pathways taken by endogenous domain-specific integral proteins of the rat hepatocyte plasma membrane in vivo. Our results suggest that both apical(More)
Information federations promise an enhanced collaboration between individual stakeholders in the life cycle of commercial products, including software and hardware products from arbitrary business sectors. However, information sharing across corporate borders must be controlled by tailored mechanisms for enforcing individual business confidentiality and(More)
The previously produced monoclonal antibody IEC 1/48 against cultured rat intestinal crypt cells (Quaroni, A., and K. J. Isselbacher. 1981. J. Natl. Cancer Inst. 67:1353-1362) was extensively characterized and found to be directed against the beta subunit of (Na+ + K+)-ATPase as assessed by immunological and enzymatic criteria. Under nondenaturing(More)
Utilizing mobile applications in the area of industrial field service promises several benefits, especially improved process support providing tailored service information and presenting it in an ad-hoc manner. The suggested approaches up to now are more or less beneficial or can’t be applied in this area at all due to some assumptions that(More)
Mobile solutions provide the opportunity for seamless integration of mobile field service engineers into theservice processes resulting in new form of interactionbetween them and the dispatcher.In this paper, we describe the results of our study regarding the requirements of utilizing a mobile applicationin the area of industrial field service. After a(More)
Novel subcellular fractionation procedures and pulse-chase techniques were used to study the intracellular transport of the microvillar membrane hydrolases sucrase-isomaltase and dipeptidylpeptidase IV in the differentiated colon adenocarcinoma cell line Caco-2. The overall rate of transport to the cell surface was two fold faster for dipeptidylpeptidase IV(More)
Murine mAbs were produced against purified microvillus membranes of rat colonocytes in order to establish a marker protein for this membrane. The majority of antibodies binding to the colonic microvillus membrane recognized a single protein with a mean apparent Mr of 120 kD in both proximal and distal colon samples. The antigen is membrane bound as probed(More)
Instant availability of up-to-date information is a vital pre-requisite in today's business for decision making and the execution of many job tasks. Especially, in service business, which is to a high degree human-based and knowledge-driven, service job experience and information about the customer as well as the installed equipment are critical success(More)
The increasing complexity of industrial products, an aging workforce, and tremendous amount of product information from enterprise information systems as well as the emerging Internet of Things are some of the challenges that have to be faced in the area of industrial field service. Mobile and semantic technologies may help to provide the right information,(More)