Bernard LW Loo

Learn More
BACKGROUND The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, but this fragmentation-based method is labor intensive. Recently, a fragmentation-free PCR based protocol has been published, termed(More)
Background Chinese hamster ovary (CHO) cells have become dominant host cells in the biopharmaceutical industry due to their capacity for proper protein folding, assembly and post-translational modifications. However, low specific productivity (qp) places limitations on yields obtained from mammalian host cells. MicroRNAs (miRNAs), a novel class of short,(More)
  • 1