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The spatial arrangement of the smooth endoplasmic reticulum (SER) was studied in 0.5-2 mum thick sections of rat spinal and chick ciliary ganglia previously impregnated with heavy metal salts. Electron microscopy at low (10-5 V) or high (10-6 V) voltage showed the impregnated SER as a continuous system extending probably from the perikaryon to the axon(More)
Subpopulations of primary sensory neurons in mammalian dorsal root ganglion (DRG) exhibit carbonic anhydrase (CA) activity. To identify these subpopulations in DRG cells of mouse and chicken, the reliability of the cytochemical localization of the enzyme requires that several conditions be fulfilled: Preservation of the enzyme activity in(More)
Radioautographs of pancreatic acinar cells were prepared in rats and mice sacrificed at various times after injection of leucine-, glycine-, or methionine-H(3). Measurements of radioactivity concentration (number of silver grains per unit area) and relative protein concentration (by microspectrophotometry of Millon-treated sections) yielded the mean(More)
The renewal of protein in retinal rods and cones has been analyzed by quantitative electron microscope radioautography in adult frogs injected with a mixture of radioactive amino acids. Protein synthesis occurs predominantly in the ergastoplasm, localized in the myoid region of the photoreceptor cells. Much of the newly formed protein next flows through the(More)
The "axonal flow" theory holds that the nerve cell body is continuously forming new cytoplasm which enters the axon and travels along its length. This theory was formulated by Weiss and collaborators (Weiss and Hiscoe, '48) to account for the accumulation of material in front of a nerve constriction and for the high concentration of enzymes appearing in the(More)
The peripheral projections of two distinct subpopulations of primary sensory neurons, expressing either calbindin D-28k or substance P, were studied in chick hindlimbs by immunodetecting calbindin D-28k with a rabbit antiserum and substance P with a mouse monoclonal antibody. Calbindin D-28k-immunoreactive axons provided an innervation restricted to(More)