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Directed migration of diverse cell types plays a critical role in biological processes ranging from development and morphogenesis to immune response, wound healing, and regeneration. However, techniques to direct, manipulate, and study cell migration in vitro and in vivo in a specific and facile manner are currently limited. We conceived of a strategy to(More)
1 Purpose This BioBricks Foundation Request for Comments (BBF RFC) describes an alternative assembly standard based on the Type IIS restriction enzyme AarI. 2 Relation to other BBF RFCs BBF RFC 28 does not update or replace any earlier BBF RFC. 4 Description We are using a multi-part/combinatorial cloning technique that is particularly well suited to(More)
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