Benjamin Pelz

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In this study we expand the accessible dynamic range of single-molecule force spectroscopy by optical tweezers to the microsecond range by fast sampling. We are able to investigate a single molecule for up to 15 min and with 300-kHz bandwidth as the protein undergoes tens of millions of folding/unfolding transitions. Using equilibrium analysis and(More)
Actin-binding proteins (ABPs) regulate the assembly of actin filaments (F-actin) into networks and bundles that provide the structural integrity of the cell. Two of these ABPs, filamin and alpha-actinin, have been extensively used to model the mechanical properties of actin networks grown in vitro; however, there is a lack in the understanding of how the(More)
Mechanical forces are important signals for cell response and development, but detailed molecular mechanisms of force sensing are largely unexplored. The cytoskeletal protein filamin is a key connecting element between the cytoskeleton and transmembrane complexes such as integrins or the von Willebrand receptor glycoprotein Ib. Here, we show using(More)
Actin cytoskeleton has long been a focus of attention due to its biological significance and unique rheological properties. Although F-actin networks have been extensively studied experimentally and several theoretical models proposed, the detailed molecular interactions between actin binding proteins (ABPs) and actin filaments that regulate network(More)
Spontaneous folding of a polypeptide chain into a knotted structure remains one of the most puzzling and fascinating features of protein folding. The folding of knotted proteins is on the timescale of minutes and thus hard to reproduce with atomistic simulations that have been able to reproduce features of ultrafast folding in great detail. Furthermore, it(More)
The regulation of protein function through ligand-induced conformational changes is crucial for many signal transduction processes. The binding of a ligand alters the delicate energy balance within the protein structure, eventually leading to such conformational changes. In this study, we elucidate the energetic and mechanical changes within the subdomains(More)
Enzymes are molecular machines that bind substrates specifically, provide an adequate chemical environment for catalysis and exchange products rapidly, to ensure fast turnover rates. Direct information about the energetics that drive conformational changes is difficult to obtain. We used subnanometre single-molecule force spectroscopy to study the energetic(More)
Degradation of n-alkanes by Cunninghamella echinulata, Absidia glauca and Mucor sp. is effected by monoterminal oxidation of the alkane chain and production of a cellular fatty-acid pattern that depends on the given n-alkane. Primary and secondary alcohols, ketones and aldehydes were not excreted in course of the oxidation. Subterminal attack on the alkane(More)
The ab i l i ty of molds to ut i l ize h y d r o c a r b o n s ha s long been k n o w n [1 ], y e t l i t t l e is k n o w n a b o u t t he m e c h a n i s m s involved. I n a r ecen t pape r [2] we showed t h a t ke tone fo rma t ion is no t a p rope r ty of Penicillia alone, b u t can be a t t r i b u t e d to severa l A@ergilli, imper fec t fung i and(More)
1. Six strains of alkane assimilating molds (belonging to the order of the Mucorales and Moniliales) were isolated from 120 different samples of soil, taken from the parks of Muenster. The soil had not been contacted or enriched with crude oil or paraffinic substances before. These strains plus ten strains from our own collection were tested for their(More)