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Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals
Enzymes from extremely thermophilic microorganisms have been of technological interest for some time because of their ability to catalyze reactions of industrial significance at elevatedExpand
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Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2‐based 3‐hydroxypropionate production
Metabolically engineered strains of the hyperthermophile Pyrococcus furiosus (Topt 95–100°C), designed to produce 3‐hydroxypropionate (3HP) from maltose and CO2 using enzymes from the MetallosphaeraExpand
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Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3‐hydroxypropionate by metabolically engineered Pyrococcus furiosus
Acetyl‐Coenzyme A carboxylase (ACC), malonyl‐CoA reductase (MCR), and malonic semialdehyde reductase (MRS) convert HCO3− and acetyl‐CoA into 3‐hydroxypropionate (3HP) in theExpand
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Determinants of Sulfur Chemolithoautotrophy in the Extremely Thermoacidophilic Sulfolobales.
Species in the archaeal order Sulfolobales thrive in hot acid and exhibit remarkable metabolic diversity. Some species are chemolithoautotrophic, obtaining energy through oxidation of inorganicExpand
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Biotechnology of extremely thermophilic archaea.
Although the extremely thermophilic archaea (Topt ≥ 70°C) may be the most primitive extant forms of life, they have been studied to a limited extent relative to mesophilic microorganisms. Many ofExpand
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Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
ABSTRACT n-Butanol is generated as a natural product of metabolism by several microorganisms, but almost all grow at mesophilic temperatures. A synthetic pathway for n-butanol production from acetylExpand
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Extremely thermophilic energy metabolisms: biotechnological prospects.
New strategies for metabolic engineering of extremely thermophilic microorganisms to produce bio-based fuels and chemicals could leverage pathways and physiological features resident in extremeExpand
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Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms
The current upper thermal limit for life as we know it is approximately 120°C. Microorganisms that grow optimally at temperatures of 75°C and above are usually referred to as ‘extreme thermophiles’Expand
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A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum
One potential advantage of an extremely thermophilic metabolic engineering host (T opt ≥ 70°C) is facilitated recovery of volatile chemicals from the vapor phase of an active fermenting culture. ThisExpand
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