Benjamín Garzón

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In vivo fetal rat stomach produces HCl 48 h before birth. This study examines the mechanisms of H+ secretion from days 19 to 21 before birth. Isolated fetal stomachs were mounted as flat sheets in Ussing chambers for measurement of the transepithelial H+ fluxes (JH+) and short-circuit current (Isc), as indexes of the active ionic fluxes, and for measurement(More)
The responsiveness of fetal rat stomach to the main agonists and antagonists of acid secretion was examined in vivo on days 19 and 20 of gestation. Significant acidification of the gastric content was observed only in response to pentagastrin, gastrin G-17, histamine and carbamylcholine from day 20, when the fetal stomach starts secreting hydrochloric acid.(More)
The pH of gastric fluid was measured in rat fetuses during the last 3 days of gestation. On day 19, the gastric pH was close to neutral. During the night of day 20, the pH was clearly lowered (6.11 +/- 0.15 units), this decrease becoming more marked on the following day. At birth (day 22), just before the first feeding, the pH of gastric fluid reached the(More)
Rat stomach sensitivity to pentagastrin was examined in fetal (days 19--21) and newborn (5--day-old) preparations in vivo and in vitro. Gastric acidification in vivo was expressed as the gastric content pH and in vitro as the net transepithelial H+ fluxes determined in an Ussing chamber. In both preparations, fetal stomach first responded to pentagastrin on(More)
Gastric acid secretion was studied in anesthetized rats from day 6 of the postnatal period up to the time of weaning. Basal H+ secretion was detected from day 6 in the first group studied (2.4 +/- 0.2 muEq of H+/10 min/100 g of body weight, BW) and remained constant up to the time of weaning (day 18: 2.5 +/- 0.2 muEq of H+/10 min/100 g of body weight)(More)
Gastric H+-K+-ATPase activity was examined in subcellular fractions (P1, 1,000 g; P2, 20,000 g; P3, 100,000 g) obtained from fundic mucosae of fetal, newborn, and adult rats. Depending on the tissue secretory state the distribution of this enzyme activity predominates in P3, i.e., resting adult, or in P2, i.e., stimulated adult, while total enzyme activity(More)
1. Unidirectional and net Na+ and Cl- fluxes were determined across isolated fetal rat stomach 19-21 days post-coitum, and across the stomach of newborn rats aged 5 or 12 days. 2. On fetal day 19, absorption of both Na+ and Cl- was greater than the short-circuit current, Isc (net Na+ flux, JnetNa = 4.7 +/- 1.0 and net Cl- flux, JnetCl = 5.4 +/- 1.4 mu equiv(More)
This study examined the presence of substance P and calcitonin-gene-related peptide (CGRP) immunoreactivities in various milks and infant formulas. Rat milk was obtained from lactating dams between parturition and weaning (0, 2, 5, 10, 15, and 20 d postpartum). Samples of human milk were obtained from seven multiparous, nonsmoking white women, and newborn(More)
The substantia nigra (SN), the subthalamic nucleus (STN), and the red nucleus (RN) are midbrain structures of ample interest in many neuroimaging studies, which may benefit from the availability of automated segmentation methods. The high iron content of these structures awards them high contrast in quantitative susceptibility mapping (QSM) images. We(More)
A bovine milk diet (BM) resulted in remarkable changes in histamine H2 receptor activity (sensitization) and PGE2 receptor activity (desensitization) in gastric glands isolated from adult rats. In contrast, the receptor-cAMP systems sensitive to glucagon(s) and secretin in parietal cells and muco-peptic cells were unaffected. In the two experimental groups,(More)