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The ripening physiology of detached fruit is altered by low oxygen conditions with profound effects on quality parameters. To study hypoxia-related processes and regulatory mechanisms, apple (Malus domestica, cv Granny Smith) fruit, harvested at commercial ripening, were kept at 1°C under normoxic (control) and hypoxic (0.4 and 0.8 kPa oxygen) conditions(More)
Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner cell layers, tissues and organs still represents a major challenge for immunolocalization studies. Here we describe a protocol for whole-mount(More)
In this study, a detailed characterization of earthworm low molecular size humic substances (LMS) was performed and these substances were used to study their effect on the nitrate influx in roots, tissue nitrate content, and expression of maize genes putatively involved in nitrate uptake in maize (Zea mays L.). The results show that the humic fraction with(More)
Two peach genes homologous to the Arabidopsis ethylene receptor genes ETR1 and ERS1, named Pp-ETR1 and Pp-ERS1 respectively, have been isolated and characterized. Pp-ETR1 and Pp-ERS1 are conserved in terms of exon numbers and intron positions, although the first and fifth introns of Pp-ETR1 have an unusual length. In addition, two putative polyadenylation(More)
During ethylene-promoted leaflet abscission in Sambucus nigra a 7-fold increase in expansin activity was detected specifically in tissues undergoing cell separation, whilst only low levels of activity were seen in adjacent non-abscising tissues. An RT-PCR strategy was used to amplify expansin fragments from a cDNA library generated from mRNA extracted from(More)
The regulation of plant growth responds to many stimuli. These responses allow environmental adaptation, thereby increasing fitness. In many cases, the relay of information about a plant's environment is through plant hormones. These messengers integrate environmental information into developmental pathways to determine plant shape. This review will use, as(More)
The function of monomeric GTPases of the RAS superfamily in fruit development and ripening has been partially characterized. Here the identification of peach (Prunus persica) small GTPases of the RAS superfamily expressed in fruit and the characterization of their expression profiles during fruit development are described. Extensive searches on expressed(More)
± transporter and of an H +-ATPase in relation to whole plant nitrate transport physiology in two maize genotypes differently responsive to low nitrogen availability Abstract Two maize genotypes differently responsive to nitrogen availability were characterized for their ef®ciency in nitrate accumulation via both the LATS (Low-Af®nity Transport System) and(More)
Biotechnological innovations that allow one to sample gene expression (e.g. gene-expression arrays or real-time Polymerase Chain Reaction (PCR)), have made it possible to measure the gene expression levels of a biological system with varying degree of accuracy, cost and speed. By repeating the measurement steps at different sampling rates during the system(More)
The MOB family includes a group of cell cycle-associated proteins highly conserved throughout eukaryotes, whose founding members are implicated in mitotic exit and co-ordination of cell cycle progression with cell polarity and morphogenesis. Here we report the characterization and evolution of the MOB domain-containing proteins as inferred from the 43(More)