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OBJECTIVE To develop a method of quality control for Hugan qingzhi tablets. METHODS Fructus Crataegi, Rhizoma Alismatis and Radix Notoginseng were identified by TLC. HPLC was used for the determination of ursolic acid in Hugan qingzhi tablets. RESULTS The chromatographic spots were identified without the interference of negative control. Ursolic acid(More)
Deep sequencing of the variable region of 16S rRNA genes has become the predominant tool for studying microbial ecology. As sequencing datasets have accumulated, meta-analysis of sequences obtained with different variable 16S rRNA gene targets and by different sequencing methods has become an intriguing prospect that remains to be evaluated experimentally.(More)
OBJECTIVE To determine the optimal extracting technique for the volatile oil from the Chinese herbs for manufacturing Xiaoerjianpixiaoshi oral solution. METHODS Two steam distillation processes were carried out to extract the volatile oil from three Chinese herbs. UV-spectrophotometry method was used to determine the oil content in the distillate, and the(More)
OBJECTIVE To establish a method for determining the content of L-sesamin and L-asarinin indifferent parts of Zanthoxylum(Roxb.)DC. METHOD High-performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18 and the mobile phase containing acetonitrile and water (50:50), the detection wavelength being 287 nm. (More)
OBJECTIVE To optimize the extraction process of Tuirezhitongsan and extract the pharmacodynamically active fractions. METHODS Orthogonal test methods and supercritical-CO(2) fluid extraction (SFE-CO(2)) were used for the extraction, and the the pharmacodynamically active fractions were screened with analgesic experiments. RESULTS The optimal extraction(More)
OBJECTIVE To investigate the effect of Hugan Qingzhi tablets on lipid metabolism and inflammation in rats fed on high-fat diet and explore the underlying mechanisms. METHODS Sixty male Sprague-Dawley rats were randomly divided into 6 groups, namely HFD group (with high-fat diet and distilled water), control group (with normal diet and distilled water),(More)
OBJECTIVE To evaluate the possibility of using the technique of CO2 supercritical fluid extraction (SFE) combined with molecular distillation (MD) to extract, separate and purify the volatile components of Ligusticum wallichii Franch, a Chinese herbal medicine. METHODS SFE was employed to extract the volatile components of Ligusticum wallichii Franch,(More)
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