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Median filtering is a cornerstone of modern image processing and is used extensively in smoothing and de-noising applications. The fastest commercial implementations (e.g. in Adobe&#174; Photoshop&#174; CS2) exhibit <i>O(r)</i> runtime in the radius of the filter, which limits their usefulness in realtime or resolution-independent contexts. We introduce a(More)
We present the results of two Hubble Space Telescope (HST) observing programs, consisting of 11 imaging observations of 10 asteroids. The primary focus of the projects was to search for faint companions (satellites) of these asteroids. No binary systems were detected. More specifically, no companions were found at more than 0.1 arcsec separation of(More)
Calf brain prolidase covalently bound to CNBr-Sepharose 4B, retained about 32% of the activity of the uncoupled enzyme. The free enzyme showed slightly greater stability than the bound preparation when stored at 20 degrees C or at 0 degrees C. However, in either case the free and bound enzymes were more stable at the lower temperature. Greater thermal(More)
Optimal conditions have been determined for the coupling of rat liver phenylalanine hydroxylase (Phe H) to cyanogen bromide-Sepharose 4B. When 8 mg of ligand was reacted with 100 mg of matrix, 20 to 30 percent of the initial enzyme activity was covalently bound along with 90 percent of the protein. The coupled enzyme showed greater thermal stability from 40(More)
We have investigated methods of stabilizing prolidase by chemical modification and covalent coupling to various supports, for use in protein hydrolysis and possible use in enzyme replacement therapy. Purified acetone powder of calf brain prolidase was further purified by gel filtration on Sephadex G-200 and chromatography on DEAE-Sephadex A25.(More)
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