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TGN38 and TGN41 are isoforms of an integral membrane protein (TGN38/41) that is predominantly localized to the trans-Golgi network (TGN) of normal rat kidney cells. Polyclonal antisera to TGN38/41 have been used to monitor its appearance at, and removal from, the surface of control and Brefeldin A (BFA)-treated cells. Antibodies that recognize the lumenal(More)
We address the problem of automatic word boundary detection in quiet and in the presence of noise. Attention has been given to automatic word boundary detection for both additive noise and noise-induced changes in the talker's speech production (Lombard reflex). After a comparison of several automatic word boundary detection algorithms in different(More)
Brefeldin A (BFA) has a dramatic effect on the morphology of the Golgi apparatus and induces a rapid redistribution of Golgi proteins into the ER (Lippincott-Schwartz, J., L. C. Yuan, J. S. Bonifacino, and R. D. Klausner. 1989. Cell. 56:801-813). To date, no evidence that BFA affects the morphology of the trans-Golgi network (TGN) has been presented. We(More)
TGN38 is an integral membrane protein previously shown to be predominantly localized to the trans-Golgi network (TGN) of cells by virtue of a signal contained within its cytoplasmic 'tail' [Luzio, Brake, Banting, Howell, Braghetta & Stanley (1990) Biochem. J. 270, 97-102]. We now (i) describe the isolation of cDNA clones encoding an isoform of TGN38, (ii)(More)
This study examines whether characteristics of a car voice can affect driver performance and affect. In a 2 (driver emotion: happy or upset) x 2 (car voice emotion: energetic vs. subdued) experimental study, participants (N=40) had emotion induced through watching one of two sets of 5-minute video clips. Participants then spent 20 minutes in a driving(More)
Key concerns of automobile interface designers are driving performance and safety. As cars include voices for telematics, command and control, warning messages, etc., these voices become an opportunity to affect drivers and their performance. In this experimental study, participants (N=36) spent 20 minutes in a driving simulator. The car presented randomly(More)
TGN38/41 is an integral membrane protein which recycles between the trans-Golgi network (TGN) and the cell surface but is predominantly located in the TGN of rat (NRK) cells at steady state. As part of our studies on the mechanism and route of recycling between the TGN and the cell surface we have used chloroquine or Bafilomycin A1 to modulate the lumenal(More)
—Accurate location of the endpoints of an isolated word is important for reliable and robust word recognition. The endpoint detection problem is nontrivial for nonstationary backgrounds where arti-facts (i.e., nonspeech events) may be introduced by the speaker, the recording environment, and the transmission system. Several techniques for the detection of(More)
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