Beatriz Souto Pérez

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A procedure was developed to rapidly isolate functional, intact mitochondria from turkey spermatozoa. Semen was collected from turkeys, pooled, and centrifuged to remove spermiophages and other cells. The sperm cells were then mechanically disrupted with a Dounce homogenizer, sonicated, and centrifuged using a discontinuous Percoll gradient. Electron(More)
Reactions of benzil bis(thiosemicarbazone), LH(6), with M(NO(3))(2).nH(2)O (M = Zn, Cd, and Ni), in the presence of LiOH.H(2)O, show the versatile behavior of this molecule. The structure of the ligand, with the thiosemicarbazone moieties on opposite sides of the carbon backbone, changes to form complexes by acting as a chelating molecule. Complexes of(More)
Spermiophages were isolated from turkey semen using Percoll gradient centrifugation, cultured in Roswell Park Memorial Institute 1640 medium at room temperature, and characterized by transmission electron microscopy. After 1 to 2 h, the cells enlarged and developed numerous motile mitochondria. Over time, the mitochondria appeared to increase in number and(More)
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