Beáta Holečková

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Gastroenteritis is one of the most frequent microbial diseases, which is caused by the ingestion of food contaminated with staphylococcal enterotoxins. In our study, the production of staphylococcal enterotoxins A, B (SEA, SEB) and the presence of respective staphylococcal enterotoxin genes were investigated in the field S. aureus isolates obtained from(More)
Adverse effects associated with occupational exposure to benzene have often been reported in humans. It has been shown, that benzene causes chromosomal aberrations, sister chromatid exchanges and micronuclei in lymphocytes of exposed workers. In addition to evidence by conventional cytogenetic methods, the genotoxic effect of benzene has also been proved by(More)
The cytogenetic effects of in vitro exposure to benzene were investigated in cultured bovine peripheral lymphocytes. Stable and unstable chromosome aberrations (CA) and sister chromatid exchanges (SCE) were examined. Cultures of lymphocytes from two healthy donors were treated with benzene at concentrations of 5, 10, 50, 100, 500 and 1000 micromol.l-1 for(More)
The potential for genotoxic and cytotoxic effects of tolylfluanid-based fungicide (50% active agent) was evaluated using sister chromatid exchange (SCE) and proliferation indices (PI) in cultured bovine peripheral lymphocytes. For the detection of possible genetic damage, DNA fragmentation assay was also applied. Bovine lymphocytes cultured for 72 h were(More)
The effect of the fungicide Euparen Multi (containing 50% tolylfluanid) was investigated on the induction of chromosomal aberrations (CA) in cultured bovine peripheral lymphocytes. Cultures from two healthy donors were treated with tolylfluanid-based fungicide at concentrations ranging from 1.7 to 17.5 μg/ml for the last 24 and 48 hours of cultivation.(More)
To date, most data about the possible genotoxic effect of triazole pesticides are focused on laboratory animals resulting in limited information on further non-target organisms such as cattle. The objective of the present study was to investigate the effect of triazole (tebuconazole/prothioconazole) fungicide formulation on the induction of chromosomal(More)
The production of staphylococcal enterotoxin A and staphylococcal enterotoxin B was investigated in S. aureus isolates obtained from milk of clinically healthy sheep. In comparison with valid standard (up to 1. 10 CFU. ml), the S. aureus counts were higher in 36 of the total number of 46 positive milk samples (ranging from 1.2. 10 to 8.2. 10 CFU. ml). Six(More)
Benzene is a relatively common environmental and occupational contaminant with carcinogenic and clastogenic properties. Therefore, further understanding of the adverse effect of benzene is still a matter of interest. In the present study, induction of aberrations in the pericentromeric region of chromosome 1 (1q12) was examined by fluorescence in situ(More)