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The purpose of this study was to determine if multiple injections of different dosages of beta-glucan derived from barley would enhance the immune response and disease resistance against infections due to opportunistic pathogens Aeromonas hydrophila and Edwardsiella tarda in Labeo rohita fingerlings. Hence, four different dosages of beta-glucan suspension(More)
Achyranthes aspera seed was incorporated in the diets (at 0.01%, 0.1% and 0.5%) of Labeo rohita, rohu fingerlings (3.0+/-0.4 g). After 2 weeks, the fish were immunized with heat-killed Aeromonas hydrophila, and after a further 2 weeks the rohu were experimentally infected with Aeromonas hydrophila (ATCC 49140). After 7 days blood and serum were sampled to(More)
The effect of exposure to sub-lethal concentrations of cypermethrin, a synthetic pyrethroid pesticide, on biochemical parameters of muscle, blood and enzyme activities in brain, liver and kidney of the Indian major carp, Labeo rohita was studied. The sub-lethal exposure studies were done for up to 45 days at 1/10 and 1/50 of 96 h LC(50) of cypermethrin. The(More)
Here we report single walled-carbon nanotubes (SWNTs)-based chemiresistor aptasensors for highly sensitive and selective detection of weakly or uncharged molecules using the displacement format. As a proof-of-concept we demonstrate the detection of ATP, a small weakly charged molecule, by displacement of the ssDNA anti-ATP aptamer hybridized to a small(More)
The expression of Mx transcripts and Mx protein was monitored at weekly intervals for 7 weeks, by qRT-PCR and immunohistochemistry, in the kidney, liver, gill and blood of Atlantic salmon parr following injection of poly I:C. Elevated levels of Mx transcripts compared to PBS injected control fish were found in the tissues at week 1. Background levels were(More)
A polyclonal rabbit antiserum directed against the conserved region of the Atlantic salmon antiviral Mx1 protein was used to detect the putative Atlantic cod Mx protein using Western and dot blotting. A doublet band at about 75 kDa and 65 kDa was detected by Western blotting in kidney and spleen extracts of cod 3 and 4 days after i.p. injection with poly(More)
The study evaluated the efficacy of dietary doses of Mangifera indica (mango) kernel on the immune response and disease resistance of Labeo rohita fingerlings against the bacterial pathogen Aeromonas hydrophila infections in. L. rohita fingerlings fed diet containing 0 (Control), 1g, 5 g, 10 g mango kernel kg(-1) dry diet for 60 days. Biochemical (serum(More)
A histopathological study of carp (Labeo rohita) ABSTRACT Indian major carp (Labeo rohita) were exposed to 1/10 and 1/5 sub-lethal doses of hexachlorocyclohexane during a 45-day trial period. Fish were sacrificed at the end of the trial period to study light microscopic changes associated with toxicity. Organ tissues, viz., liver, kidney, gill, skin,(More)
Atlantic cod fry (1g) were infected by intraperitoneal injection with IPNV and samples of liver were taken every second day from four fish up to day 21. Samples were analysed for levels of viral transcripts by real time RT-PCR and the induction of expression of interferon stimulated gene 15 (ISG15) transcripts were estimated by conventional RT-PCR relative(More)