Learn More
This study investigates alginate-chitosan polyelectrolyte complexes (PECs) in the form of a film, a precipitate, as well as a layer-by-layer (LbL) assembly. The focus of this study is to fully characterize, using the complementary techniques of Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) in combination with(More)
A rapid, sensitive and reproducible gas chromatographic method with flame ionization detection is described for the simultaneous identification and quantification of 33 amino acids and dipeptides in spent cell culture media in under seven minutes. The method involves the use of the EZ:faast(Phenomenex) amino acid sample testing kit. Instrumental and assay(More)
A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed. In order to more tightly control glycosylation profiles, we sought to identify the cause of variability. Untargeted metabolomics and transcriptomics methods(More)
Raw materials are a critical part of any cell culture medium; therefore, it is of utmost importance to understand and characterize them for high-quality product. The raw material characterization (RMC) program at SAFC focuses on individual screening of raw materials both analytically and biologically. The goal of the program is to develop the best-in-class(More)
  • 1