Learn More
An optical biosensor for urea measurements was developed. The operation of the sensor is based on the well-known urease enzyme-catalyzed hydrolysis of urea. The ammonium ions liberated in the reaction are detected with an ion selective optode membrane containing nonactin as ion selective ionophore and ETH 5294 chromoionophore in a thin (1 microm)(More)
EXECUTIVE SUMMARY The evolution of e-Government services moves quickly. There is a limited time for adaptation to the new environment in terms of legislation, society, and economy. Maintaining reliable services and a secure IT environment is even more difficult with perpetual changes like mergers and acquisitions, supply chain activity, staff turnover, and(More)
e-Government services have to operate in dynamic environments, and there is a limited time for adaptation in terms of legislation, society, and economy. Maintaining reliable services is even more difficult with continuous changes, like mergers and acquisitions, supply chain activity, staff turnover, and regulatory variation. The nature of the changes has(More)
Public administration institutions – as well as citizens and businesses – have to meet challenges of the constantly changing business and legal environment. The complexity and quantity of information to be faced with by these actors is increasing at an alarming rate. Research and development projects must turn to the development of innovative, modern(More)
An opto-electrochemical sensor for copper (II) ion was developed. The sensor consists of a planar conductive indium-tin-oxide (ITO) glass support, which was coated by a polymeric copper (II) sensitive membrane. The sensing membrane is made of plasticized polyurethane matrix containing Zincon colorimetric reagent immobilized as ion pair with(More)
Three generations of alpha,gamma-diaminobutyric acid modified poly(propyleneimine) dendrimers [DAB(AM)n, n = 4, 8, 16] containing 4, 8, 16 free amino groups were coupled with Boc-protected alpha,gamma-diaminobutyric acid (DABA) moieties in high yields. These modified dendrimers were deprotected and the chiral dendritic amines with 8, 16 and 32 amino groups(More)
Scanning electrochemical microscopy (SECM) with amperometric or potentiometric measuring tips was used to investigate biocatalytic reactions inside the enzyme layer of a biosensor during its operation. The well known glucose oxidase catalyzed oxidation of glucose has been selected for the studies. Local, instantaneous concentration of dissolved oxygen and(More)