Barbora Křížová

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BACKGROUND Polymerase chain reaction (PCR) has become a common technique offering fast and sensitive analysis of DNA in food/feed samples. However, many substances, either already present in the sample or introduced during sample processing, inhibit PCR and thus underestimate the DNA content. It is therefore necessary to identify PCR inhibition in order to(More)
Sovová T., Křížová B., Drábková L., Ovesná J. (2017): Detection of PCR inhibition in food and feed with a synthetic plasmid. Czech J. Food Sci., 35: 160–164. We present a successful use of the plasmid inhibition detection and DNA isolation protocol optimisation for four food/feed samples in qPCR analysis of the sequence coding for chloroplast tRNA-Leu: two(More)
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