Barbara Reinhold

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The populations of diazotrophic and nondiazotrophic bacteria were estimated in the endorhizosphere and on the rhizoplane of Kallar grass (Leptochloa fusca) and in nonrhizosphere soil. Microaerophilic diazotrophs were counted by the most-probable-number method, using two semisolid malate media, one of them adapted to the saline-sodic Kallar grass soil. Plate(More)
Chemotactic responses of three Azospirillum strains originating from different host plants were compared to examine the possible role of chemotaxis in the adaptation of these bacteria to their respective hosts. The chemotaxis to several sugars, amino acids, and organic acids was determined qualitatively by an agar plate assay and quantitatively by a(More)
F. Ardellier I. Barabanov J. C. Barrière F. Beißel S. Berridge L. Bezrukov A. Bernstein T. Bolton N.S. Bowden Ch. Buck B. Bugg J. Busenitz A. Cabrera E. Caden C. Cattadori S. Cazaux M. Cerrada B. Chevis H. Cohn J. Coleman S. Cormon B. Courty A. Cucoanes M. Cribier N. Danilov S. Dazeley A. Di Vacri Y. Efremenko A. Etenko M. Fallot C. Fernández-Bedoya F. von(More)
A mixture of ananain (EC 3.4.22.31) and comosain purified from crude pineapple stem extract was found to contain numerous closely related enzyme forms. Chromatographic separation of the major enzyme forms was achieved after treatment of the mixture with thiol-modifying reagents: reversible modification with 2-hydroxyethyl disulphide provided enzyme for(More)
The effect of oxygen on N(2)-dependent growth of two Azospirillum strains and two diazotrophic rods closely associated with roots of Kallar grass (Leptochloa fusca) was studied. To enable precise comparison, bacteria were grown in dissolved-oxygen-controlled batch and continuous cultures. Steady states were obtained from about 1 to 30 muM O(2), some of them(More)
Diazotrophic rods occurring in high numbers (about 10 rods per g [dry weight] of root) in the root interior of Kallar grass were localized by indirect immunofluorescence in cross sections of the same roots. Specifically stained round bodies which were apparently covered by a nonantigenic envelope were found in the aerenchymatic tissue.
There is increasing evidence that nitrogen-fixing bacteria are able to colonize the interior of grass roots. Several techniques have been used to demonstrate the sites of colonization and are discussed. Among the techniques useful for specific labelling, gold-labelled reagents have been frequently successfully applied in other fields. We propose the use of(More)
Bacteria occurring in high numbers on the rhizoplane of kallar grass grown at a natural site in Pakistan were effective scavengers of traces of combined nitrogen from the atmosphere. Bacteria grew under appropriate conditions in nitrogen-free semi-solid malate medium in the form of a typical subsurface pellicle which resulted in a significant nitrogen gain(More)
Purified bovine erythrocyte acetylcholinesterase (AChE) was radiomethylated on its amine groups and incubated with bacterial phosphatidylinositol-specific phospholipase C to remove the lipid portion of the AChE glycoinositol phospholipid (GPI) anchor, and a C-terminal tryptic fragment that contained the residual GPI glycan was isolated by HPLC. Analysis by(More)