Bao-qian Jing

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AIM To investigate the sphingosine 1-phosphate (S1P) receptor expression profile in human esophageal cancer cells and the effects of S1P5 on proliferation and migration of human esophageal cancer cells. METHODS S1P receptor expression profile in human esophageal squamous cell carcinoma cell line Eca109 was detected by semi-quantitative reverse(More)
OBJECTIVE To explore the protein profile and identify developmentally regulated proteins of the promastigotes and axenic amastigotes with comparative proteomics technique. METHODS The total proteins of promastigotes and axenic amastigotes of Leishmania donovani SC6 strain were separated by two-dimensional electrophoresis (2-DE) in a broad pH range (3-10),(More)
OBJECTIVE To investigate the expression level of virulence-associated genes in promastigotes and amastigotes of different Leishmania spp. METHODS Total RNA was extracted from the promastigotes and amastigotes of Leishmania donovani, L. infantum, L. tropica, L. major and L. mexicana, and relevant strains. According to the reported gene sequences in(More)
OBJECTIVE To establish a molecular marking method to identify Pinellia ternata and Typhonium flagelliforme. METHODS Twenty-two random oligonucleotide primers were used in RAPD analysis on the genomic DNA of two types of Pinellia ternata in Sichuan and two types of Typhonium flagelliforme in Guangxi. The special fragments were sequenced, marked as probes(More)
OBJECTIVE To clone the novel gene that specifically expressed in the amastigotes of Leishmania donovani, and observe subcellular localization of the gene encoding protein. METHODS mRNA from promastigotes and amastigotes of L. donovani were prepared. The novel expressed sequence tag of amastigotes was selected by suppression subtractive hybridization. The(More)
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